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Literature summary extracted from

  • Cross, J.V.; Rady, J.M.; Foss, F.W.; Lyons, C.E.; Macdonald, T.L.; Templeton, D.J.
    Nutrient isothiocyanates covalently modify and inhibit the inflammatory cytokine macrophage migration inhibitory factor (MIF) (2009), Biochem. J., 423, 315-321.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.3.12 recombinant expression of MIF in HeLa cells and in Escherichia coli strain BL21(DE3) Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
5.3.3.12 additional information preparation of biotin-isothiocyanate-labeled enzyme, proteomic screening in recombinant HeLa cells and identification of the modification site by mass spectrometry Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.3.12 Isothiocyanate inhibits tautomerase activity of MIF. Modification and inhibition of MIF is not involved in the induction of phase 2 gene expression in response to isothiocyanate treatment Homo sapiens
5.3.3.12 phenethylisothiocyanate inhibits tautomerase activity of MIF Homo sapiens
5.3.3.12 sulforaphane inhibits tautomerase activity of MIF Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.3.12 additional information Homo sapiens MIF is a 12 kDa protein with an enzymatic keto-enol tautomerase activityMIF is a homotrimeric multifunctional proinflammatory cytokine. Binding of MIF to its receptor, CD74, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.12 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.3.12 L-3,4-dihydroxyphenylalanine methyl ester i.e. L-3,4-dihydroxyphenylalanine methyl ester hydrochloride, the catalytic residue is N-terminal Pro1 Homo sapiens methyl 3-(3,4-dioxocyclohexa-1,5-dien-1-yl)-L-alaninate
-
 r
5.3.3.12 additional information MIF is a 12 kDa protein with an enzymatic keto-enol tautomerase activityMIF is a homotrimeric multifunctional proinflammatory cytokine. Binding of MIF to its receptor, CD74, overview Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.3.12 D-dopachrome tautomerase
-
 Homo sapiens
5.3.3.12 Macrophage migration inhibitory factor
-
 Homo sapiens
5.3.3.12 MIF
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
5.3.3.12 0.001 0.002
-
 Homo sapiens sulforaphane
5.3.3.12 0.005 0.006
-
 Homo sapiens phenethylisothiocyanate