EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.2.1 | expressed in Escherichia coli BL21(DE3) cells | Geobacillus stearothermophilus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.1.2.1 | mutant proteins are crystallized by the hanging drop vapor diffusion method, using 50% 2-methyl 2,4-pentanediol as the precipitant with 0.2 mM EDTA, 5 mM beta-mercaptoethanol in 100 mM HEPES (pH 7.5) | Geobacillus stearothermophilus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.2.1 | F351G | the mutation has no effect on tetrahydrofolate-independent and tetrahydrofolate-dependent activities | Geobacillus stearothermophilus |
2.1.2.1 | N341A | the mutant is inactive for the tetrahydrofolate-dependent activity, while the mutation has no effect on tetrahydrofolate-independent activity | Geobacillus stearothermophilus |
2.1.2.1 | Y60A | the mutant is inactive for the tetrahydrofolate-dependent activity, while the mutation has no effect on tetrahydrofolate-independent activity | Geobacillus stearothermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.1 | 0.9 | - |
L-serine | wild type enzyme, at 37°C | Geobacillus stearothermophilus | |
2.1.2.1 | 1 | - |
L-serine | mutant enzyme F315G, at 37°C | Geobacillus stearothermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.2.1 | Geobacillus stearothermophilus | Q7SIB6 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.2.1 | ammonium sulfate precipitation, DEAE-cellulose column chromatography, gel filtration | Geobacillus stearothermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.1 | L-serine + tetrahydrofolate | - |
Geobacillus stearothermophilus | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
2.1.2.1 | additional information | SHMT also catalyses several tetrahydrofolate-independent side reactions such as cleavage of beta-hydroxy amino acids, transamination, racemization and decarboxylation | Geobacillus stearothermophilus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.2.1 | dimer | x-ray crystallography | Geobacillus stearothermophilus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.2.1 | serine hydroxymethyltransferase | - |
Geobacillus stearothermophilus |
2.1.2.1 | SHMT | - |
Geobacillus stearothermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.2.1 | 65 | - |
the melting temperature of the wild type enzyme is at 65°C | Geobacillus stearothermophilus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.1 | 3.9 | - |
L-serine | wild type enzyme, at 37°C | Geobacillus stearothermophilus | |
2.1.2.1 | 3.9 | - |
L-serine | mutant enzyme F315G, at 37°C | Geobacillus stearothermophilus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.2.1 | pyridoxal 5'-phosphate | dependent on | Geobacillus stearothermophilus |