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Literature summary extracted from

  • Vidal, I.; Gonzalez, J.; Bernardo, A.; Martin, R.
    Purification and classification of diacetyl-reducing enzymes from Staphylococcus aureus (1988), Biochem. J., 251, 461-466.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.304 diacetyl substrate inhibition at concentrations above 80-90 mM Staphylococcus aureus
1.1.1.304 ethyl pyruvate substrate inhibition at concentrations above 80-90 mM Staphylococcus aureus
1.1.1.304 Methyl pyruvate substrate inhibition at concentrations above 80-90 mM Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.304 0.025
-
 NADH 25°C, pH 6.0, cosubstrate 2,3-pentanedione Staphylococcus aureus
1.1.1.304 0.045
-
 NADH 25°C, pH 6.0, cosubstrate diacetyl Staphylococcus aureus
1.1.1.304 0.095
-
 NADH 25°C, pH 6.0, cosubstrate methyl pyruvate Staphylococcus aureus
1.1.1.304 0.11
-
 NADH 25°C, pH 6.0, cosubstrate ethyl pyruvate Staphylococcus aureus
1.1.1.304 6
-
 2,3-Pentanedione 25°C, pH 6.0 Staphylococcus aureus
1.1.1.304 15
-
 diacetyl 25°C, pH 6.0 Staphylococcus aureus
1.1.1.304 16
-
 Methyl pyruvate 25°C, pH 6.0 Staphylococcus aureus
1.1.1.304 24
-
 ethyl pyruvate 25°C, pH 6.0 Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.304 68000
-
 gel filtration Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.304 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.304
-
 Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.304 2,3-pentanedione + beta-NADH + H+
-
 Staphylococcus aureus L-3-hydroxy-2-pentanone + beta-NAD+
-
 ir
1.1.1.304 diacetyl + beta-NADH + H+ 86.9% of the activity with pentane-2,3-dione Staphylococcus aureus (S)-acetoin + beta-NAD+
-
 ir
1.1.1.304 ethyl pyruvate + beta-NADH + H+ 38.4% of the activity with pentane-2,3-dione Staphylococcus aureus ? + beta-NAD+
-
 ir
1.1.1.304 methyl pyruvate + beta-NADH + H+ 22.8% of the activity with pentane-2,3-dione Staphylococcus aureus ? + beta-NAD+
-
 ir
1.1.1.304 additional information no activity with alpha-NADH or NADPH Staphylococcus aureus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.304 monomer 1 * 68000, SDS-PAGE Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.304 6
-
-
 Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.304 additional information no activity with alpha-NADH or NADPH Staphylococcus aureus
1.1.1.304 NADH beta-NADH Staphylococcus aureus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.304 150
-
 ethyl pyruvate 25°C, pH 6.0 Staphylococcus aureus
1.1.1.304 150
-
 Methyl pyruvate 25°C, pH 6.0 Staphylococcus aureus
1.1.1.304 300
-
 diacetyl 25°C, pH 6.0 Staphylococcus aureus