EC Number | Cloned (Comment) | Organism |
---|---|---|
4.3.3.7 | the dapA and dapA-H225* genes introduced into the vector pUCX, to transform the auxotrophic Escherichia coli XL1-Blue KanRDELTAdapA cells, under the control of a lac promoter/repressor system. Mutations in dapA introduced into the pET-151/D-TOPO plasmid. C-terminal truncated DHDPS (H225*) expressed in Escherichia coli BL21 Star (DE3) | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.3.3.7 | additional information | C-terminal truncated DHDPS (H225*), exhibits a dramatic reduction in both solubility and stability. Equilibrating mixture of quaternary states. The substrate, pyruvate, and the feedback inhibitor, (S)-lysine, both have a positive effect on thermostability of C-terminal truncated DHDPS (H225*) | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.3.3.7 | L-lysine | - |
Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.3.7 | 0.23 | - |
L-aspartate 4-semialdehyde | C-terminal truncated DHDPS (H225*), at 30°C, in 150 mM HEPES, pH 8.0, 0.16 mM NADPH, 50 microg/ml DHDPR | Escherichia coli | |
4.3.3.7 | 0.29 | - |
L-aspartate 4-semialdehyde | polyhistidine-tagged wild-type DHDPSR | Escherichia coli | |
4.3.3.7 | 0.36 | - |
pyruvate | polyhistidine-tagged wild-type DHDPS | Escherichia coli | |
4.3.3.7 | 0.37 | - |
pyruvate | C-terminal truncated DHDPS (H225*), at 30°C, in 150 mM HEPES, pH 8.0, 0.16 mM NADPH, 50 microg/ml DHDPR | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.3.3.7 | Escherichia coli | P0A6L2 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.3.3.7 | - |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.3.7 | L-aspartate 4-semialdehyde + pyruvate | - |
Escherichia coli | dihydrodipicolinate + 2 H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.3.3.7 | DHDPS | - |
Escherichia coli |
4.3.3.7 | dihydrodipicolinate synthase | - |
Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.3.7 | 1.2 | - |
pyruvate | C-terminal truncated DHDPS (H225*), at 30°C, in 150 mM HEPES, pH 8.0, 0.16 mM NADPH, 50 microg/ml DHDPR | Escherichia coli | |
4.3.3.7 | 75 | - |
pyruvate | polyhistidine-tagged wild-type DHDPS | Escherichia coli |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
4.3.3.7 | 0.21 | - |
wild-type DHDPS | Escherichia coli | L-lysine | |
4.3.3.7 | 0.22 | - |
C-terminal truncated DHDPS (H225*) | Escherichia coli | L-lysine |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.3.3.7 | physiological function | complementation of the auxotrophy of Escherichia coli XL1-Blue KanRDELTAdapA cells only with the plasmid pUCX:dapA encoding wild-type DHDPS | Escherichia coli |