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Literature summary extracted from

  • Hsiao, Y.S.; Parker, D.; Ratner, A.J.; Prince, A.; Tong, L.
    Crystal structures of respiratory pathogen neuraminidases (2009), Biochem. Biophys. Res. Commun., 380, 467-471.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.18 expressed in Escherichia coli BL21(DE3) cells Pseudomonas aeruginosa
3.2.1.18 expressed in Escherichia coli BL21(DE3) cells Streptococcus pneumoniae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.18 free enzyme and in complex with N-acetylneuraminic acid or 2,3-dehydro-2-deoxy-N-acetylneuraminic acid, sitting drop vapor diffusion method, using 100 mM HEPES (pH 7.0) and 30% Jeffamine ED-2001 (pH 7.0), at 21°C Streptococcus pneumoniae
3.2.1.18 free enzyme, sitting drop vapor diffusion method, using 100 mM HEPES (pH 7.0), 5% tacsimate, 7% (w/v) PEG 5000MME, at 21°C Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.18 D79A inactive Pseudomonas aeruginosa
3.2.1.18 R198K inactive Pseudomonas aeruginosa
3.2.1.18 R260A inactive Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.18 2-deoxy-2,3-dehydro-N-acetyl-D-neuraminic acid
-
 Streptococcus pneumoniae
3.2.1.18 N-acetylneuraminic acid
-
 Streptococcus pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.18 Pseudomonas aeruginosa
-
-
-
3.2.1.18 Streptococcus pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.18 nickel-agarose column chromatography and gel filtration Pseudomonas aeruginosa
3.2.1.18 nickel-agarose column chromatography and gel filtration Streptococcus pneumoniae

Synonyms

EC Number Synonyms Comment Organism
3.2.1.18 NanA
-
 Streptococcus pneumoniae
3.2.1.18 NanPs
-
 Pseudomonas aeruginosa
3.2.1.18 neuraminidase
-
 Pseudomonas aeruginosa
3.2.1.18 neuraminidase
-
 Streptococcus pneumoniae