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Literature summary extracted from

  • Tasgin, E.; Nadaroglu, H.; Demir, Y.; Demir, N.
    Purification and properties of carbonic anhydrase from bone marrow (2009), Asian J. Chem., 21, 5117-5122.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.1 acetazolamide uncompetitive inhibitor Bos taurus
4.2.1.1 KSCN uncompetitive inhibitor Bos taurus
4.2.1.1 NaN3 uncompetitive inhibitor Bos taurus
4.2.1.1 sulfanilamide uncompetitive inhibitor Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.1 0.002409
-
 4-nitrophenyl acetate at pH 7.0 and 20°C Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.1 36000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.1 CO2 + H2O Bos taurus
-
 H2CO3
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1 Sepharose 4B-L-tyrosine sulfanilamide column chromatography Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.1 bone marrow
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.1 0.052
-
 crude homogenate, at 20°C Bos taurus
4.2.1.1 7.843
-
 after 150.82fold purification, at 20°C Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 4-nitrophenyl acetate + H2O
-
 Bos taurus 4-nitrophenol + acetate
-
 ?
4.2.1.1 CO2 + H2O
-
 Bos taurus H2CO3
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 carbonic anhydrase
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.1 35
-
-
 Bos taurus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.1 20 70
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.1 7
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.1 3 8
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.1 0.00000718
-
 KSCN at pH 7.0 and 20°C Bos taurus
4.2.1.1 0.0000212
-
 acetazolamide at pH 7.0 and 20°C Bos taurus
4.2.1.1 0.0000343
-
 NaN3 at pH 7.0 and 20°C Bos taurus
4.2.1.1 0.0000521
-
 sulfanilamide at pH 7.0 and 20°C Bos taurus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.2.1.1 0.824
-
 at pH 7.0 and 20°C Bos taurus sulfanilamide
4.2.1.1 1.145
-
 at pH 7.0 and 20°C Bos taurus acetazolamide
4.2.1.1 1.151
-
 at pH 7.0 and 20°C Bos taurus KSCN
4.2.1.1 1.529
-
 at pH 7.0 and 20°C Bos taurus NaN3