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Literature summary extracted from

  • Ruan, H.L.; Stec, E.; Li, S.M.
    Production of diprenylated indole derivatives by tandem incubation of two recombinant dimethylallyltryptophan synthases (2009), Arch. Microbiol., 191, 791-795.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.5.1.34 biotechnology the combination of the two dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) can be successfully used for chemoenzymatic synthesis of the diprenylated derivatives. The potential of recombinant enzymes from secondary metabolite biosynthesis as promising tools for the production of designed compounds is demonstrated Aspergillus fumigatus
2.5.1.80 biotechnology the combination of the two dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) can be successfully used for chemoenzymatic synthesis of the diprenylated derivatives. The potential of recombinant enzymes from secondary metabolite biosynthesis as promising tools for the production of designed compounds is demonstrated Aspergillus fumigatus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.34 expressed as a recombinant protein Aspergillus fumigatus
2.5.1.80 expressed as a recombinant protein Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.34 Aspergillus fumigatus
-
 two dimethylallyltryptophan synthases, FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80), which catalyse the prenylation of L-tryptophan at positions C4 and C7, respectively, are identified in Aspergillus fumigatus
-
2.5.1.80 Aspergillus fumigatus
-
 two dimethylallyltryptophan synthases, FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80), which catalyse the prenylation of L-tryptophan at positions C4 and C7, respectively, are identified in Aspergillus fumigatus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.34 dimethylallyl diphosphate + 11-methyl-DL-tryptophan tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 33.5% (FgaPT2 followed by 7-DMATS), 4.8% (7-DMATS followed by FgaPT2) Aspergillus fumigatus ?
-
 ?
2.5.1.34 dimethylallyl diphosphate + 5-methoxy-DL-tryptophan tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 10.3% (FgaPT2 followed by 7-DMATS), 3.5% (7-DMATS followed by FgaPT2) Aspergillus fumigatus ?
-
 ?
2.5.1.34 dimethylallyl diphosphate + 5-methyl-DL-tryptophan tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 24.3% (FgaPT2 followed by 7-DMATS), 0.9% (7-DMATS followed by FgaPT2) Aspergillus fumigatus ?
-
 ?
2.5.1.34 dimethylallyl diphosphate + 6-methyl-DL-tryptophan tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 2.6% (FgaPT2 followed by 7-DMATS), 1.5% (7-DMATS followed by FgaPT2) Aspergillus fumigatus ?
-
 ?
2.5.1.34 dimethylallyl diphosphate + D-tryptophan tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 4.3% (FgaPT2 followed by 7-DMATS), 2.6% (7-DMATS followed by FgaPT2) Aspergillus fumigatus ?
-
 ?
2.5.1.34 dimethylallyl diphosphate + DL-indole-3-lactic acid tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 35.7% (FgaPT2 followed by 7-DMATS), 16.7% (7-DMATS followed by FgaPT2) Aspergillus fumigatus ?
-
 ?
2.5.1.34 dimethylallyl diphosphate + L-abrine tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 92.7% (FgaPT2 followed by 7-DMATS), 11.3% (7-DMATS followed by FgaPT2) Aspergillus fumigatus ?
-
 ?
2.5.1.34 dimethylallyl diphosphate + L-beta-homotryptophan tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 31.5% (FgaPT2 followed by 7-DMATS), 6.6% (7-DMATS followed by FgaPT2) Aspergillus fumigatus ?
-
 ?
2.5.1.34 dimethylallyl diphosphate + L-tryptophan tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 89.7% (FgaPT2 followed by 7-DMATS), 20% (7-DMATS followed by FgaPT2) Aspergillus fumigatus ?
-
 ?
2.5.1.34 dimethylallyl diphosphate + tryptamine tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 11% (FgaPT2 followed by 7-DMATS), 6.3% (7-DMATS followed by FgaPT2) Aspergillus fumigatus ?
-
 ?
2.5.1.80 dimethylallyl diphosphate + 11-methyl-DL-tryptophan tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 33.5% (FgaPT2 followed by 7-DMATS), 4.8% (7-DMATS followed by FgaPT2) Aspergillus fumigatus ?
-
 ?
2.5.1.80 dimethylallyl diphosphate + 5-methoxy-DL-tryptophan tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 10.3% (FgaPT2 followed by 7-DMATS), 3.5% (7-DMATS followed by FgaPT2) Aspergillus fumigatus ?
-
 ?
2.5.1.80 dimethylallyl diphosphate + 5-methyl-DL-tryptophan tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 24.3% (FgaPT2 followed by 7-DMATS), 0.9% (7-DMATS followed by FgaPT2) Aspergillus fumigatus ?
-
 ?
2.5.1.80 dimethylallyl diphosphate + 6-methyl-DL-tryptophan tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 2.6% (FgaPT2 followed by 7-DMATS), 1.5% (7-DMATS followed by FgaPT2) Aspergillus fumigatus ?
-
 ?
2.5.1.80 dimethylallyl diphosphate + D-tryptophan tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 4.3% (FgaPT2 followed by 7-DMATS), 2.6% (7-DMATS followed by FgaPT2) Aspergillus fumigatus ?
-
 ?
2.5.1.80 dimethylallyl diphosphate + DL-indole-3-lactic acid tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 35.7% (FgaPT2 followed by 7-DMATS), 16.7% (7-DMATS followed by FgaPT2) Aspergillus fumigatus ?
-
 ?
2.5.1.80 dimethylallyl diphosphate + L-abrine tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 92.7% (FgaPT2 followed by 7-DMATS), 11.3% (7-DMATS followed by FgaPT2) Aspergillus fumigatus ?
-
 ?
2.5.1.80 dimethylallyl diphosphate + L-beta-homotryptophan tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 31.5% (FgaPT2 followed by 7-DMATS), 6.6% (7-DMATS followed by FgaPT2) Aspergillus fumigatus ?
-
 ?
2.5.1.80 dimethylallyl diphosphate + L-tryptophan tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 89.7% (FgaPT2 followed by 7-DMATS), 20% (7-DMATS followed by FgaPT2) Aspergillus fumigatus ?
-
 ?
2.5.1.80 dimethylallyl diphosphate + tryptamine tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 11% (FgaPT2 followed by 7-DMATS), 6.3% (7-DMATS followed by FgaPT2) Aspergillus fumigatus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.34 dimethylallyltryptophan synthase
-
 Aspergillus fumigatus
2.5.1.34 FgaPT2
-
 Aspergillus fumigatus
2.5.1.80 7-DMATS
-
 Aspergillus fumigatus
2.5.1.80 dimethylallyltryptophan synthase
-
 Aspergillus fumigatus