Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bertoldi, M.; Voltattorni, C.B.
    Multiple roles of the active site lysine of Dopa decarboxylase (2009), Arch. Biochem. Biophys., 488, 130-139.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.28 mutant enzyme K303A is expressed in Escherichia coli SVS370 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.28 K303A the mutant binds pyridoxal 5'-phosphate with a 100fold decreased apparent equilibrium binding affinity with respect to the wild type enzyme. Unlike the wild-type, K303A in the presence of L-Dopa displays a parallel progress course of formation of both dopamine and 3,4-dihydroxyphenylacetaldehyde (plus ammonia) with a burst followed by a linear phase Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.28 L-Dopa slight substrate inhibition is observed at high concentration of L-Dopa Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.28 0.000095
-
 L-Dopa dopamine formation, mutant enzyme K303A, in 50 mM HEPES, pH 7.5, at 25°C Homo sapiens
4.1.1.28 0.00083
-
 dopamine mutant enzyme K303A, in 50 mM HEPES, pH 7.5, at 25°C Homo sapiens
4.1.1.28 0.00092
-
 L-Dopa aldehyde formation, mutant enzyme K303A, in 50 mM HEPES, pH 7.5, at 25°C Homo sapiens
4.1.1.28 0.0505
-
 dopamine wild type enzyme, in 50 mM HEPES, pH 7.5, at 25°C Homo sapiens
4.1.1.28 4.27
-
 L-Dopa wild type enzyme, in 50 mM HEPES, pH 7.5, at 25°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.28 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.28 Discovery C18 column chromatography, gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.28 L-Dopa
-
 Homo sapiens dopamine + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.28 DDC
-
 Homo sapiens
4.1.1.28 DOPA decarboxylase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.28 0.07
-
 L-Dopa wild type enzyme, in 50 mM HEPES, pH 7.5, at 25°C Homo sapiens
4.1.1.28 0.11
-
 L-Dopa aldehyde formation, mutant enzyme K303A, in 50 mM HEPES, pH 7.5, at 25°C Homo sapiens
4.1.1.28 0.188
-
 L-Dopa dopamine formation, mutant enzyme K303A, in 50 mM HEPES, pH 7.5, at 25°C Homo sapiens
4.1.1.28 0.7
-
 dopamine mutant enzyme K303A, in 50 mM HEPES, pH 7.5, at 25°C Homo sapiens
4.1.1.28 2.48
-
 dopamine wild type enzyme, in 50 mM HEPES, pH 7.5, at 25°C Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.28 pyridoxal 5'-phosphate
-
 Homo sapiens