EC Number | Application | Comment | Organism |
---|---|---|---|
2.3.2.2 | analysis | analytical method for the selective determination and speciation of S-nitrosoglutathione and its metabolite S-nitrosocysteinylglycine, based on liquid chromatography separation coupled to on-line enzymatic hydrolysis of S-nitrosoglutathione by commercial GGT. The limit of quantitation for S-nitrosoglutathione and S-nitrosocysteinylglycine in plasma ultrafiltrate is 5 nM, with a precision of 1-6% within the 5-1500 nM dynamic linear range. The method is applied to evaluate the recovery of exogenous S-nitrosoglutathione after addition of aliquots to human plasma samples presenting with different total GGT activities. By inhibiting GGT activity in a time-dependent manner, the recovery of S-nitrosoglutathione is inversely correlated with plasmatic levels of endogenous GGT | Bos taurus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.2.2 | Bos taurus | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.3.2.2 | commercial preparation | - |
Bos taurus | - |
2.3.2.2 | kidney | - |
Bos taurus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.2.2 | S-nitroso-glutathione + glycyl-glycine | - |
Bos taurus | S-nitroso-cysteinylglycine + gamma-L-glutamyl-glycyl-glycine | - |
? |