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Literature summary extracted from
- Gene cloning, protein characterization, and alteration of product selectivity for the trehalulose hydrolase and trehalulose synthase from "Pseudomonas mesoacidophila" MX-45 (2009), Appl. Environ. Microbiol., 75, 7026-7036.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.11 | expressed in Escherichia coli JM109 cells | Paraburkholderia acidicola |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.4.99.11 | D442N | the mutant favors the transfer reaction with an isomer preference for isomaltulose | Paraburkholderia acidicola |
| 5.4.99.11 | R311C | the mutant demonstrates higher catalytic efficiency for D-glucose production over trehalulose production | Paraburkholderia acidicola |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.11 | 30 | - |
Trehalulose | wild type enzyme | Paraburkholderia acidicola |  |
| 5.4.99.11 | 30 | - |
isomaltulose | wild type enzyme | Paraburkholderia acidicola | |
| 5.4.99.11 | 40 | - |
isomaltulose | mutant enzyme R311C | Paraburkholderia acidicola | |
| 5.4.99.11 | 43 | - |
D-glucose | mutant enzyme R311C | Paraburkholderia acidicola | |
| 5.4.99.11 | 79 | - |
Trehalulose | mutant enzyme R311C | Paraburkholderia acidicola | |
| 5.4.99.11 | 100 | - |
D-glucose | mutant enzyme D442N | Paraburkholderia acidicola | |
| 5.4.99.11 | 185 | - |
Trehalulose | mutant enzyme D442N | Paraburkholderia acidicola | |
| 5.4.99.11 | 240 | - |
D-glucose | wild type enzyme | Paraburkholderia acidicola | |
| 5.4.99.11 | 500 | - |
isomaltulose | mutant enzyme D442N | Paraburkholderia acidicola | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 5.4.99.11 | periplasm | - |
Paraburkholderia acidicola | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.11 | 55000 | - |
SDS-PAGE | Paraburkholderia acidicola |
| 5.4.99.11 | 63750 | - |
MALDI-TOF mass spectrometry | Paraburkholderia acidicola |
| 5.4.99.11 | 64290 | - |
mature MutB, calculated from amino acid sequence | Paraburkholderia acidicola |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.11 | Paraburkholderia acidicola | Q2PS28 | - |
- |
| 5.4.99.11 | Paraburkholderia acidicola MX-45 | Q2PS28 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.11 | - |
Paraburkholderia acidicola |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.11 | sucrose | MutB catalyzes the isomerization of sucrose to mainly trehalulose (1-O-alpha-D-glucopyranosyl-beta-D-fructose) | Paraburkholderia acidicola | isomaltulose + trehalulose + D-glucose | - |
r | |
| 5.4.99.11 | sucrose | MutB catalyzes the isomerization of sucrose to mainly trehalulose (1-O-alpha-D-glucopyranosyl-beta-D-fructose) | Paraburkholderia acidicola MX-45 | isomaltulose + trehalulose + D-glucose | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.11 | MutB | - |
Paraburkholderia acidicola |
| 5.4.99.11 | sucrose isomerase | - |
Paraburkholderia acidicola |
| 5.4.99.11 | Trehalulose synthase | - |
Paraburkholderia acidicola |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.11 | 1.7 | - |
isomaltulose | mutant enzyme R311C | Paraburkholderia acidicola | |
| 5.4.99.11 | 11.5 | - |
Trehalulose | mutant enzyme R311C | Paraburkholderia acidicola | |
| 5.4.99.11 | 15 | - |
D-glucose | wild type enzyme | Paraburkholderia acidicola | |
| 5.4.99.11 | 32.5 | - |
D-glucose | mutant enzyme R311C | Paraburkholderia acidicola | |
| 5.4.99.11 | 44 | - |
D-glucose | mutant enzyme D442N | Paraburkholderia acidicola | |
| 5.4.99.11 | 241 | - |
Trehalulose | mutant enzyme D442N | Paraburkholderia acidicola | |
| 5.4.99.11 | 258 | - |
isomaltulose | wild type enzyme | Paraburkholderia acidicola | |
| 5.4.99.11 | 1144 | - |
Trehalulose | wild type enzyme | Paraburkholderia acidicola | |
| 5.4.99.11 | 1947 | - |
isomaltulose | mutant enzyme D442N | Paraburkholderia acidicola | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.11 | 0.042 | - |
isomaltulose | mutant enzyme R311C | Paraburkholderia acidicola | |
| 5.4.99.11 | 0.06 | - |
D-glucose | wild type enzyme | Paraburkholderia acidicola | |
| 5.4.99.11 | 0.15 | - |
Trehalulose | mutant enzyme R311C | Paraburkholderia acidicola | |
| 5.4.99.11 | 0.44 | - |
D-glucose | mutant enzyme D442N | Paraburkholderia acidicola | |
| 5.4.99.11 | 0.76 | - |
D-glucose | mutant enzyme R311C | Paraburkholderia acidicola | |
| 5.4.99.11 | 1.3 | - |
Trehalulose | mutant enzyme D442N | Paraburkholderia acidicola | |
| 5.4.99.11 | 3.9 | - |
isomaltulose | mutant enzyme D442N | Paraburkholderia acidicola | |
| 5.4.99.11 | 8.6 | - |
isomaltulose | wild type enzyme | Paraburkholderia acidicola | |
| 5.4.99.11 | 36 | - |
Trehalulose | wild type enzyme | Paraburkholderia acidicola | |
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