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Literature summary extracted from

  • Palomo, M.; Kralj, S.; van der Maarel, M.J.; Dijkhuizen, L.
    The unique branching patterns of Deinococcus glycogen branching enzymes are determined by their N-terminal domains (2009), Appl. Environ. Microbiol., 75, 1355-1362.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.18 expression in Escherichia coli BL21 Deinococcus geothermalis
2.4.1.18 expression in Escherichia coli BL21 Deinococcus radiodurans

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.18 additional information CT Dg, truncated at 3' end Deinococcus geothermalis
2.4.1.18 additional information CTT Dr, tuncated at 3' end Deinococcus radiodurans
2.4.1.18 additional information GGR, construction of chimeric genes, with C-domain of GBE of Deinococcus radiodurans Deinococcus geothermalis
2.4.1.18 additional information GGR, construction of chimeric genes, with N-domain and A-domain of GBE of Deinococcus geothermalis Deinococcus radiodurans
2.4.1.18 additional information GRR, construction of chimeric genes, with A-domain and C-domain of GBE of Deinococcus radiodurans Deinococcus geothermalis
2.4.1.18 additional information GRR, construction of chimeric genes, with N-domain of GBE of Deinococcus geothermalis Deinococcus radiodurans
2.4.1.18 additional information NT Dg, tuncated at 5' end Deinococcus geothermalis
2.4.1.18 additional information RGG, construction of chimeric genes, with A-domain and C-domain of GBE of Deinococcus geothermalis Deinococcus radiodurans
2.4.1.18 additional information RGG, construction of chimeric genes, with N-domain of GBE of Deinococcus radiodurans Deinococcus geothermalis
2.4.1.18 additional information RRG, construction of chimeric genes, with C-domain of GBE of Deinococcus geothermalis Deinococcus radiodurans
2.4.1.18 additional information RRG, construction of chimeric genes, with N-domain and A-domain of GBE of Deinococcus radiodurans Deinococcus geothermalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.18 soluble
-
 Deinococcus geothermalis
-
-
2.4.1.18 soluble
-
 Deinococcus radiodurans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.18 75000
-
 SDS-PAGE Deinococcus geothermalis
2.4.1.18 80000
-
 SDS-PAGE Deinococcus radiodurans

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.18 Deinococcus geothermalis Q1IZQ3 strain DSM 11300
-
2.4.1.18 Deinococcus radiodurans Q9RTB7
-
-
2.4.1.18 Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 Q9RTB7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.18 His tag affinity chromatography Deinococcus geothermalis
2.4.1.18 His tag affinity chromatography Deinococcus radiodurans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.18 additional information
-
 specific activity on amylopectin 342.3 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant RGG, iodine assay Deinococcus geothermalis
2.4.1.18 additional information
-
 specific activity on amylopectin 342.3 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant RGG, iodine assay Deinococcus radiodurans
2.4.1.18 additional information
-
 specific activity on amylopectin 359.2 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant GGR, iodine assay Deinococcus geothermalis
2.4.1.18 additional information
-
 specific activity on amylopectin 359.2 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant GGR, iodine assay Deinococcus radiodurans
2.4.1.18 additional information
-
 specific activity on amylopectin 474.3 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, wild-type enzyme, iodine assay Deinococcus geothermalis
2.4.1.18 additional information
-
 specific activity on amylopectin 505.6 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant RRG, iodine assay Deinococcus geothermalis
2.4.1.18 additional information
-
 specific activity on amylopectin 505.6 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant RRG, iodine assay Deinococcus radiodurans
2.4.1.18 additional information
-
 specific activity on amylopectin 538.0 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, wild-type enzyme, iodine assay Deinococcus radiodurans
2.4.1.18 additional information
-
 specific activity on amylopectin 556.5 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant CTT Dr, iodine assay Deinococcus radiodurans
2.4.1.18 additional information
-
 specific activity on amylopectin less than 0.5 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant GRR, iodine assay Deinococcus geothermalis
2.4.1.18 additional information
-
 specific activity on amylopectin less than 0.5 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant GRR, iodine assay Deinococcus radiodurans
2.4.1.18 additional information
-
 specific activity on amylose 404.2 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, wild-type enzyme, iodine assay Deinococcus radiodurans
2.4.1.18 additional information
-
 specific activity on amylose 411.7 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant RRG, iodine assay Deinococcus geothermalis
2.4.1.18 additional information
-
 specific activity on amylose 411.7 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant RRG, iodine assay Deinococcus radiodurans
2.4.1.18 additional information
-
 specific activity on amylose 442.3 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant CTT Dr, iodine assay Deinococcus radiodurans
2.4.1.18 additional information
-
 specific activity on amylose 468.8 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant GGR, iodine assay Deinococcus geothermalis
2.4.1.18 additional information
-
 specific activity on amylose 468.8 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant GGR, iodine assay Deinococcus radiodurans
2.4.1.18 additional information
-
 specific activity on amylose 621.3 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, wild-type enzyme, iodine assay Deinococcus geothermalis
2.4.1.18 additional information
-
 specific activity on amylose 624.1 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant RGG, iodine assay Deinococcus geothermalis
2.4.1.18 additional information
-
 specific activity on amylose 624.1 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant RGG, iodine assay Deinococcus radiodurans
2.4.1.18 additional information
-
 specific activity on amylose less than 0.5 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant GRR, iodine assay Deinococcus geothermalis
2.4.1.18 additional information
-
 specific activity on amylose less than 0.5 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant GRR, iodine assay Deinococcus radiodurans
2.4.1.18 4.7
-
 wild-type enzyme, branching assay Deinococcus radiodurans
2.4.1.18 7.2
-
 wild-type enzyme, branching assay Deinococcus geothermalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.18 amylopectin iodine assay Deinococcus geothermalis ?
-
 ?
2.4.1.18 amylopectin iodine assay Deinococcus radiodurans ?
-
 ?
2.4.1.18 amylopectin iodine assay Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 ?
-
 ?
2.4.1.18 amylose branching assay, at pH 8.0, reaction stopped by boiling for 5 min Deinococcus geothermalis ?
-
 ?
2.4.1.18 amylose branching assay, at pH 8.0, reaction stopped by boiling for 5 min Deinococcus radiodurans ?
-
 ?
2.4.1.18 amylose branching assay, at pH 8.0, reaction stopped by boiling for 5 min Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.18 1,4-alpha-glucan branching enzyme
-
 Deinococcus geothermalis
2.4.1.18 1,4-alpha-glucan branching enzyme
-
 Deinococcus radiodurans
2.4.1.18 Dg GBE
-
 Deinococcus geothermalis
2.4.1.18 Dr GBE
-
 Deinococcus radiodurans
2.4.1.18 GH13 GBE
-
 Deinococcus geothermalis
2.4.1.18 GH13 GBE
-
 Deinococcus radiodurans
2.4.1.18 glycogen branching enzyme
-
 Deinococcus geothermalis
2.4.1.18 glycogen branching enzyme
-
 Deinococcus radiodurans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.18 34
-
 iodine assay Deinococcus geothermalis
2.4.1.18 34
-
 iodine assay Deinococcus radiodurans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.18 30 40 iodine assay Deinococcus geothermalis
2.4.1.18 30 40 iodine assay Deinococcus radiodurans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.18 50
-
 after 1 h incubation at fully active Deinococcus radiodurans
2.4.1.18 60 65 after 1 h incubation at 60°C fully active, after 1 h incubation at 65°C only 80% residual activity Deinococcus geothermalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.18 8
-
 iodine assay Deinococcus geothermalis
2.4.1.18 8
-
 iodine assay Deinococcus radiodurans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.18 7 9 iodine assay Deinococcus geothermalis
2.4.1.18 7 9 iodine assay Deinococcus radiodurans