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Literature summary extracted from

  • Hoelsch, K.; Havel, J.; Haslbeck, M.; Weuster-Botz, D.
    Identification, cloning, and characterization of a novel ketoreductase from the cyanobacterium Synechococcus sp. strain PCC 7942 (2008), Appl. Environ. Microbiol., 74, 6697-6702.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.100 additional information variation of the buffer molarity between 25 mM and 200 mM or addition of NaCl up to 500 mM has no significant effect on enzyme activity Synechococcus elongatus PCC 7942 = FACHB-805

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.100 transformation of the fabG gene encoding 3-ketoacyl-[acyl-carrier-protein] reductase in fusion with a His6-MBP-tag (ligated into vector pETM-41) into Escherichia coli XL-10 Gold. After isolation from XL-10 cells and DNA sequencing, transformed into Escherichia coli Tuner (DE3) cells Synechococcus elongatus PCC 7942 = FACHB-805

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.100 1.92
-
 ethylbenzoylacetate with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
1.1.1.100 2.71
-
 alpha-chloroacetophenone with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
1.1.1.100 5.72
-
 2',3',4',5',6'-pentafluoroacetophenone with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
1.1.1.100 6.03
-
 propiophenone with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
1.1.1.100 7.38
-
 3'-fluoroacetophenone with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
1.1.1.100 8.31
-
 ethyl 4-chloroacetoacetate with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
1.1.1.100 12.29
-
 4'-fluoroacetophenone with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
1.1.1.100 14.34
-
 4'-chloroacetophenone with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
1.1.1.100 46.92
-
 acetophenone with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
1.1.1.100 89.2
-
 1,1,1-trifluoroacetone with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.100 25500
-
 4 * 25500, SDS-PAGE Synechococcus elongatus PCC 7942 = FACHB-805
1.1.1.100 102000
-
 gel filtration Synechococcus elongatus PCC 7942 = FACHB-805

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.100 Synechococcus elongatus PCC 7942 = FACHB-805 Q31QF3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.100 by gel filtration and ion exchange chromatography, to homogeneity Synechococcus elongatus PCC 7942 = FACHB-805

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.100 0.02
-
 with 2-octanone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
1.1.1.100 0.08
-
 with 4'-fluoroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
1.1.1.100 0.13
-
 with 3'-fluoroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
1.1.1.100 0.13
-
 with acetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
1.1.1.100 0.15
-
 with 4'-chloroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
1.1.1.100 0.16
-
 with propiophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
1.1.1.100 1.03
-
 with 1,1,1-trifluoroacetone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
1.1.1.100 1.16
-
 with ethylbenzoylacetate as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
1.1.1.100 2.45
-
 with alpha-chloroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
1.1.1.100 3.93
-
 with 2',3',4',5',6'-pentafluoroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
1.1.1.100 38.29
-
 with ethyl 4-chloroacetoacetate as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.100 1,1,1-trifluoroacetone + NADPH 792% relative activity compared to acetophenone Synechococcus elongatus PCC 7942 = FACHB-805 ? + NADP+
-
 ?
1.1.1.100 2',3',4',5',6'-pentafluoroacetophenone + NADPH 3023% relative activity compared to acetophenone Synechococcus elongatus PCC 7942 = FACHB-805 ? + NADP+
-
 ?
1.1.1.100 2-octanone + NADPH 15% relative activity compared to acetophenone Synechococcus elongatus PCC 7942 = FACHB-805 ? + NADP+
-
 ?
1.1.1.100 3'-fluoroacetophenone + NADPH 100% relative activity compared to acetophenone Synechococcus elongatus PCC 7942 = FACHB-805 ? + NADP+
-
 ?
1.1.1.100 4'-chloroacetophenone + NADPH 115% relative activity compared to acetophenone Synechococcus elongatus PCC 7942 = FACHB-805 ? + NADP+
-
 ?
1.1.1.100 4'-fluoroacetophenone + NADPH 62% relative activity compared to acetophenone Synechococcus elongatus PCC 7942 = FACHB-805 ? + NADP+
-
 ?
1.1.1.100 acetophenone + NADPH 100% relative activity Synechococcus elongatus PCC 7942 = FACHB-805 ? + NADP+
-
 ?
1.1.1.100 alpha-chloroacetophenone + NADPH 1885% relative activity compared to acetophenone Synechococcus elongatus PCC 7942 = FACHB-805 ? + NADP+
-
 ?
1.1.1.100 ethyl 4-chloroacetoacetate + NADPH 29454% relative activity compared to acetophenone Synechococcus elongatus PCC 7942 = FACHB-805 ? + NADP+
-
 ?
1.1.1.100 ethylbenzoylacetate + NADPH 892% relative activity compared to acetophenone Synechococcus elongatus PCC 7942 = FACHB-805 ? + NADP+
-
 ?
1.1.1.100 propiophenone + NADPH 123% relative activity compared to acetophenone Synechococcus elongatus PCC 7942 = FACHB-805 ? + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.100 tetramer 4 * 25500, SDS-PAGE Synechococcus elongatus PCC 7942 = FACHB-805

Synonyms

EC Number Synonyms Comment Organism
1.1.1.100 3-ketoacyl-[acyl-carrier-protein] reductase
-
 Synechococcus elongatus PCC 7942 = FACHB-805

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.100 44
-
-
 Synechococcus elongatus PCC 7942 = FACHB-805

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.100 30 55 30°C: about 55% of maximal activity, 55°C: about 65% of maximal activity Synechococcus elongatus PCC 7942 = FACHB-805

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.100 8
-
-
 Synechococcus elongatus PCC 7942 = FACHB-805

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.100 4 10 pH 4: about 60% of maximal activity, pH 10: about 75% of maximal activity Synechococcus elongatus PCC 7942 = FACHB-805