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Literature summary extracted from

  • Frade-Perez, M.D.; Hernandez-Cervantes, A.; Flores-Carreon, A.; Mora-Montes, H.M.
    Biochemical characterization of Candida albicans alpha-glucosidase I heterologously expressed in Escherichia coli (2010), Antonie van Leeuwenhoek, 98, 291-298.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.20 expression in Escherichia coli Candida albicans
3.2.1.106 expressed in Escherichia coli BL21(DE3) cells Candida albicans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.20 1-deoxynojirimycin
-
 Candida albicans
3.2.1.20 australine 0.02 mM, 39% inhibition of enzyme activity Candida albicans
3.2.1.20 bromoconduritol 0.02 mM, 22% inhibition of enzyme acitvity Candida albicans
3.2.1.20 castanospermine
-
 Candida albicans
3.2.1.20 D-glucose
-
 Candida albicans
3.2.1.20 D-mannose
-
 Candida albicans
3.2.1.20 N-ethylmaleimide
-
 Candida albicans
3.2.1.106 1-deoxynojirimycin
-
 Candida albicans
3.2.1.106 australine 39% inhibition at 0.02 mM Candida albicans
3.2.1.106 bromoconduritol 22% inhibition at 0.02 mM Candida albicans
3.2.1.106 castanospermine
-
 Candida albicans
3.2.1.106 D-glucose
-
 Candida albicans
3.2.1.106 mannose
-
 Candida albicans
3.2.1.106 additional information 2-deoxyglucose, 3-deoxyglucose or 6-deoxyglucose do not have any effect on the enzyme activity; the enzyme is completely insensitive to diethyl pyrocarbonate Candida albicans
3.2.1.106 N-ethylmaleimide
-
 Candida albicans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 0.133
-
 4-Methylumbelliferyl-alpha-D-glucopyranoside
-
 Candida albicans
3.2.1.106 0.133
-
 4-methylumbelliferyl alpha-D-glucopyranoside in 10 mM sodium phosphate buffer pH 7.0 and at 37°C Candida albicans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.20 endoplasmic reticulum
-
 Candida albicans 5783
-
3.2.1.106 endoplasmic reticulum
-
 Candida albicans 5783
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 50000
-
 SDS-PAGE, recombinant enzyme Candida albicans
3.2.1.106 47800
-
 subunit, calculated from amino acid sequence Candida albicans
3.2.1.106 50000
-
 subunit, SDS-PAGE Candida albicans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.106 Glc3Man9GlcNAc2 + H2O Candida albicans alpha-glucosidase I removes the outermost alpha1,2-glucose unit from the N-linked core Glc3Man9GlcNAc2 D-glucose + Glc2Man9GlcNAc2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Candida albicans Q2V621
-
-
3.2.1.106 Candida albicans Q2V621
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 anion exchange chromatography in DEAE Bio-Gel, Sephadex G-25 column Candida albicans
3.2.1.106 DEAE Bio-Gel A column chromatography and Sephadex G-25 gel filtration Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 4-methylumbelliferyl-alpha-D-glucopyranoside + H2O assay at pH 7.0, 37°C, 30 min incubation Candida albicans 4-methylumbelliferone + alpha-D-glucose
-
 ?
3.2.1.106 4-methylumbelliferyl alpha-D-glucopyranoside + H2O not a common substrate for alpha-glucosidase I Candida albicans 4-methylumbelliferone + alpha-D-glucopyranose
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O alpha-glucosidase I removes the outermost alpha1,2-glucose unit from the N-linked core Glc3Man9GlcNAc2 Candida albicans D-glucose + Glc2Man9GlcNAc2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.20 alpha-glucosidase I
-
 Candida albicans
3.2.1.20 CWH41
-
 Candida albicans
3.2.1.106 alpha-glucosidase I
-
 Candida albicans
3.2.1.106 Cwh41p
-
 Candida albicans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 37
-
 assay at Candida albicans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 7
-
 assay at Candida albicans
3.2.1.106 6 7 recombinant enzyme, in 10 mM sodium phosphate buffer and at 37°C Candida albicans

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.20 0.0027
-
-
 Candida albicans castanospermine
3.2.1.20 0.0029
-
-
 Candida albicans 1-deoxynojirimycin
3.2.1.20 2.2
-
-
 Candida albicans N-ethylmaleimide
3.2.1.20 2.9
-
-
 Candida albicans D-glucose
3.2.1.20 9.1
-
-
 Candida albicans D-mannose
3.2.1.106 0.0027
-
 in 10 mM sodium phosphate buffer pH 7.0 and at 37°C Candida albicans castanospermine
3.2.1.106 0.0029
-
 in 10 mM sodium phosphate buffer pH 7.0 and at 37°C Candida albicans 1-deoxynojirimycin
3.2.1.106 2.2
-
 in 10 mM sodium phosphate buffer pH 7.0 and at 37°C Candida albicans N-ethylmaleimide
3.2.1.106 2.9
-
 in 10 mM sodium phosphate buffer pH 7.0 and at 37°C Candida albicans D-glucose
3.2.1.106 9.1
-
 in 10 mM sodium phosphate buffer pH 7.0 and at 37°C Candida albicans mannose

General Information

EC Number General Information Comment Organism
3.2.1.106 physiological function alpha-glucosidase I is required for normal cell wall composition, and virulence of Candida albicans Candida albicans