Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Vecchione, J.J.; Sello, J.K.
    A novel tryptophanyl-tRNA synthetase gene confers high-level resistance to indolmycin (2009), Antimicrob. Agents Chemother., 53, 3972-3980.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
6.1.1.2 medicine as producers of nearly half of the 10000 known antibiotics, Streptomyces bacteria have been particularly important in drug discovery and are a major source of drug resistance genes Streptomyces coelicolor
6.1.1.2 medicine as producers of nearly half of the 10000 known antibiotics, Streptomyces bacteria have been particularly important in drug discovery and are a major source of drug resistance genes Streptomyces griseus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.2 the vectors pMS81, pIJ10257, and pBluescript KS+ are used Streptomyces coelicolor

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.2 TrpRS2(H48N) ORF mutant, constructed for analysing the indolmycin resistance Streptomyces coelicolor
6.1.1.2 TrpRS2(H48Q) ORF mutant, constructed for analysing the indolmycin resistance Streptomyces coelicolor
6.1.1.2 TrpRS2(L9F) ORF mutant, constructed for analysing the indolmycin resistance Streptomyces coelicolor
6.1.1.2 TrpRS2(L9F/H48Q) ORF mutant, constructed for analysing the indolmycin resistance Streptomyces coelicolor
6.1.1.2 TrpRS2(L9F/Q13K) ORF mutant, constructed for analysing the indolmycin resistance Streptomyces coelicolor
6.1.1.2 TrpRS2(Q13K) ORF mutant, constructed for analysing the indolmycin resistance Streptomyces coelicolor
6.1.1.2 TrpRS2[C(-17)A/H48Q] locus mutant, constructed for analysing the indolmycin resistance Streptomyces coelicolor
6.1.1.2 TrpRS2[C(-17)A/L9F] locus mutant, constructed for analysing the indolmycin resistance Streptomyces coelicolor
6.1.1.2 TrpRS2[C(-17)A] locus mutant, constructed for analysing the indolmycin resistance Streptomyces coelicolor
6.1.1.2 TrpRS2[G(-13)T/H48Q] locus mutant, constructed for analysing the indolmycin resistance Streptomyces coelicolor
6.1.1.2 TrpRS2[G(-13)T/L9F] locus mutant, constructed for analysing the indolmycin resistance Streptomyces coelicolor
6.1.1.2 TrpRS2[G(-13)T] locus mutant, constructed for analysing the indolmycin resistance Streptomyces coelicolor

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.2 additional information indolmycin, a potential antibacterial drug, completely inhibits bacterial tryptophanyl-tRNA synthetases, identifying indolmycin resistance genes leads to the discovery of a gene encoding an indolmycin-resistant isoform of tryptophanyl-tRNA synthetase Streptomyces coelicolor
6.1.1.2 additional information indolmycin, a potential antibacterial drug, completely inhibits bacterial tryptophanyl-tRNA synthetases, identifying indolmycin resistance genes leads to the discovery of a gene encoding an indolmycin-resistant isoform of tryptophanyl-tRNA synthetase Streptomyces griseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.2 ATP + L-tryptophan + tRNATrp Streptomyces coelicolor
-
 AMP + diphosphate + L-tryptophyl-tRNATrp
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp Streptomyces griseus
-
 AMP + diphosphate + L-tryptophyl-tRNATrp
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.2 Streptomyces coelicolor
-
-
-
6.1.1.2 Streptomyces griseus B1VQY7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.2 ATP + L-tryptophan + tRNATrp
-
 Streptomyces coelicolor AMP + diphosphate + L-tryptophyl-tRNATrp
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp
-
 Streptomyces griseus AMP + diphosphate + L-tryptophyl-tRNATrp
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.2 TrpRS2
-
 Streptomyces coelicolor
6.1.1.2 TrpRS2
-
 Streptomyces griseus
6.1.1.2 Tryptophanyl-tRNA synthetase
-
 Streptomyces coelicolor
6.1.1.2 Tryptophanyl-tRNA synthetase
-
 Streptomyces griseus

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.2 ATP
-
 Streptomyces coelicolor
6.1.1.2 ATP
-
 Streptomyces griseus