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Literature summary extracted from

  • Kitao, T.; Miyoshi-Akiyama, T.; Kirikae, T.
    AAC(6)-Iaf, a novel aminoglycoside 6-N-acetyltransferase from multidrug-resistant Pseudomonas aeruginosa clinical isolates (2009), Antimicrob. Agents Chemother., 53, 2327-2334.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.82 into the vectors pSTV28 and pQE2, pQE2 is used for expression of the protein in Escherichia coli BL21DE3 pLysS cells Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.82 21000
-
 determined by SDS-PAGE and Western Blot analysis Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.82 Pseudomonas aeruginosa C4TGI5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.82 recombinant AAC(6')-Iaf is purified using Ni-nitrilotriacetic acid agarose, native AAC(6')-Iaf from Pseudomonas aeruginosa on an anti-AAC(6')-Iaf-IgG coupled and NHS-activated Sepharose column Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.82 acetyl-CoA + gentamicin
-
 Pseudomonas aeruginosa CoA + 6'-N-acetylgentamicin
-
 ?
2.3.1.82 acetyl-CoA + neomycin
-
 Pseudomonas aeruginosa CoA + N6'-acetylneomycin
-
 ?
2.3.1.82 additional information lividomycin A is also a substrate, although with extremely small activity, thus the enzyme has acetylation activity with alternate amino groups in aminoglycosides Pseudomonas aeruginosa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.82 AAC(6')-Iaf
-
 Pseudomonas aeruginosa
2.3.1.82 aminoglycoside 6'-N-acetyltransferase
-
 Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.82 acetyl-CoA
-
 Pseudomonas aeruginosa