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Literature summary extracted from
- A continuous assay for alpha-methylacyl-coenzyme A racemase using circular dichroism (2010), Anal. Biochem., 398, 45-51.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 5.1.99.4 | medicine | alpha-methylacyl-coenzyme A racemase is a biomarker for prostate cancer and a putative target for the development of therapeutic agents directed against the disease, inhibition of enzyme activity may also act synergistically with androgen ablation therapy | Mycobacterium tuberculosis |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.1.99.4 | the open reading frame encoding alpha-methylacyl-coenzyme A racemase from Mycobacterium tuberculosis is subcloned into a pET15b vector, and the enzyme is overexpressed in Escherichia coli cells | Mycobacterium tuberculosis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.99.4 | (2R)-ibuprofenoyl-CoA | competitive inhibitor | Mycobacterium tuberculosis |  |
| 5.1.99.4 | (2S)-ibuprofenoyl-CoA | competitive inhibitor | Mycobacterium tuberculosis | |
| 5.1.99.4 | octyl-beta-D-glucopyranoside | concentrations greater than 0.6% octyl-beta-D-glucopyranoside cause a reduction in enzyme activity | Mycobacterium tuberculosis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.99.4 | 0.048 | - |
(2R)-ibuprofenoyl-CoA | recombinant enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
| 5.1.99.4 | 0.071 | - |
(2R)-ibuprofenoyl-CoA | recombinant six-His tagged enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
| 5.1.99.4 | 0.086 | - |
(2S)-ibuprofenoyl-CoA | recombinant enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
| 5.1.99.4 | 0.087 | - |
(2S)-ibuprofenoyl-CoA | recombinant six-His tagged enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 5.1.99.4 | peroxisome | - |
Mycobacterium tuberculosis | 5777 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.1.99.4 | 38970 | - |
calculated for recombinant enzyme without six-His tag | Mycobacterium tuberculosis |
| 5.1.99.4 | 40850 | - |
calculated for recombinant enzyme with six-His tag | Mycobacterium tuberculosis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.99.4 | Mycobacterium tuberculosis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.1.99.4 | by using metal ion affinity chromatography | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.99.4 | (2R)-ibuprofenoyl-CoA | - |
Mycobacterium tuberculosis | (2S)-ibuprofenoyl-CoA | - |
r | |
| 5.1.99.4 | (2R)-pristanoyl-CoA | - |
Mycobacterium tuberculosis | (2S)-pristanoyl-CoA | - |
r | |
| 5.1.99.4 | 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl-CoA | - |
Mycobacterium tuberculosis | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.1.99.4 | alpha-methylacyl-coenzyme A racemase | - |
Mycobacterium tuberculosis |
| 5.1.99.4 | AMACR | - |
Mycobacterium tuberculosis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.99.4 | 228 | - |
(2R)-ibuprofenoyl-CoA | recombinant six-His tagged enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
| 5.1.99.4 | 291 | - |
(2R)-ibuprofenoyl-CoA | recombinant enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
| 5.1.99.4 | 358 | - |
(2S)-ibuprofenoyl-CoA | recombinant six-His tagged enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
| 5.1.99.4 | 450 | - |
(2S)-ibuprofenoyl-CoA | recombinant enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.1.99.4 | metabolism | beta-oxidation pathway of fatty acids | Mycobacterium tuberculosis |
| 5.1.99.4 | physiological function | enzyme catalyzes the 1,1-proton transfer reaction that effects the reversible epimerization of the coenzyme A thioesters derived from a variety of (2R)- and (2S)-methyl branched fatty acids | Mycobacterium tuberculosis |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.99.4 | additional information | - |
additional information | Mycobacterium tuberculosis enzyme expressed as a fusion protein bearing an N-terminal six-His tag has a catalytic efficiency that is reduced 22% and 47% in the 2S-2R and 2R-2S directions, respectively, relative to untagged enzyme | Mycobacterium tuberculosis | |
| 5.1.99.4 | 0.0032 | - |
(2R)-ibuprofenoyl-CoA | recombinant six-His tagged enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
| 5.1.99.4 | 0.0041 | - |
(2S)-ibuprofenoyl-CoA | recombinant six-His tagged enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
| 5.1.99.4 | 0.0052 | - |
(2S)-ibuprofenoyl-CoA | recombinant enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
| 5.1.99.4 | 0.0061 | - |
(2R)-ibuprofenoyl-CoA | recombinant enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
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