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Literature summary extracted from

  • Bhuiya, M.W.; Liu, C.J.
    A cost-effective colorimetric assay for phenolic O-methyltransferases and characterization of caffeate 3-O-methyltransferases from Populus trichocarpa (2009), Anal. Biochem., 384, 151-158.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.68 expressed in Escherichia coli BL21(DE3) cells Populus trichocarpa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.68 0.01013
-
 3,4-dihydroxy-trans-cinnamate isoform COMT2, in 50 mM Tris-HCl, pH 7.5, at 30°C Populus trichocarpa
2.1.1.68 0.02874
-
 3,4-dihydroxy-trans-cinnamate isoform COMT1, in 50 mM Tris-HCl, pH 7.5, at 30°C Populus trichocarpa

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.68 Populus trichocarpa A9P830 black cottonwood
-
2.1.1.68 Populus trichocarpa C6FFB6 black cottonwood
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.68 Ni2+-agarose affinity column chromatography, gel filtration Populus trichocarpa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.68 stem
-
 Populus trichocarpa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.68 S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
-
 Populus trichocarpa S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
 ?
2.1.1.68 S-adenosyl-L-methionine + 5-hydroxyferulic acid
-
 Populus trichocarpa S-adenosyl-L-homocysteine + sinapic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.68 caffeate 3-O-methyltransferase
-
 Populus trichocarpa
2.1.1.68 caffeate/5-hydroxyferulate 3/5-O-methyltransferase
-
 Populus trichocarpa
2.1.1.68 COMT1 isoform Populus trichocarpa
2.1.1.68 COMT2 isoform Populus trichocarpa