BRENDA - Enzyme Database

Enolase from Klebsiella pneumoniae and human muscle cells. I. Purification and comparative molecular studies

Bednarz-Misa, I.; Pietkiewicz, J.; Bana?, T.; Gamian, A.; Adv. Clin. Exp. Med. 18, 71-78 (2009)
No PubMed abstract available

Data extracted from this reference:

Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
dependent on
Homo sapiens
4.2.1.11
Mg2+
dependent on
Klebsiella pneumoniae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
45000
-
2 * 45000, SDS-PAGE
Homo sapiens
4.2.1.11
47000
-
2 * 47000, SDS-PAGE
Klebsiella pneumoniae
4.2.1.11
94000
-
native enzyme, gel filtration
Klebsiella pneumoniae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Homo sapiens
-
-
-
4.2.1.11
Klebsiella pneumoniae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
ammonium sulfate precipitation, DEAE Sephadex A-50 gel filtration, Sephadex G-100 gel filtration
Klebsiella pneumoniae
4.2.1.11
ammonium sulfate precipitation, DEAE-Sephadex A-50 gel filtration, CM-Sephadex gel filtration, and QAE-Sephadex gel filtration
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
muscle
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
0.41
-
crude extract, at pH 7.8
Klebsiella pneumoniae
4.2.1.11
0.9
-
crude extract, at pH 6.8
Homo sapiens
4.2.1.11
31.2
-
after 76.1fold purification, at pH 7.8
Klebsiella pneumoniae
4.2.1.11
75
-
after 83fold purification, at pH 6.8
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
701579
Homo sapiens
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
-
701579
Klebsiella pneumoniae
phosphoenolpyruvate + H2O
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
homodimer
2 * 45000, SDS-PAGE
Homo sapiens
4.2.1.11
homodimer
2 * 47000, SDS-PAGE
Klebsiella pneumoniae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
dependent on
Homo sapiens
4.2.1.11
Mg2+
dependent on
Klebsiella pneumoniae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
45000
-
2 * 45000, SDS-PAGE
Homo sapiens
4.2.1.11
47000
-
2 * 47000, SDS-PAGE
Klebsiella pneumoniae
4.2.1.11
94000
-
native enzyme, gel filtration
Klebsiella pneumoniae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
ammonium sulfate precipitation, DEAE Sephadex A-50 gel filtration, Sephadex G-100 gel filtration
Klebsiella pneumoniae
4.2.1.11
ammonium sulfate precipitation, DEAE-Sephadex A-50 gel filtration, CM-Sephadex gel filtration, and QAE-Sephadex gel filtration
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
muscle
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
0.41
-
crude extract, at pH 7.8
Klebsiella pneumoniae
4.2.1.11
0.9
-
crude extract, at pH 6.8
Homo sapiens
4.2.1.11
31.2
-
after 76.1fold purification, at pH 7.8
Klebsiella pneumoniae
4.2.1.11
75
-
after 83fold purification, at pH 6.8
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
701579
Homo sapiens
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
-
701579
Klebsiella pneumoniae
phosphoenolpyruvate + H2O
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
homodimer
2 * 45000, SDS-PAGE
Homo sapiens
4.2.1.11
homodimer
2 * 47000, SDS-PAGE
Klebsiella pneumoniae