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Literature summary extracted from

  • Bednarz-Misa, I.; Pietkiewicz, J.; Bana?, T.; Gamian, A.
    Enolase from Klebsiella pneumoniae and human muscle cells. I. Purification and comparative molecular studies (2009), Adv. Clin. Exp. Med., 18, 71-78.
No PubMed abstract available

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.11 Mg2+ dependent on Homo sapiens
4.2.1.11 Mg2+ dependent on Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.11 45000
-
 2 * 45000, SDS-PAGE Homo sapiens
4.2.1.11 47000
-
 2 * 47000, SDS-PAGE Klebsiella pneumoniae
4.2.1.11 94000
-
 native enzyme, gel filtration Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Homo sapiens
-
-
-
4.2.1.11 Klebsiella pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.11 ammonium sulfate precipitation, DEAE Sephadex A-50 gel filtration, Sephadex G-100 gel filtration Klebsiella pneumoniae
4.2.1.11 ammonium sulfate precipitation, DEAE-Sephadex A-50 gel filtration, CM-Sephadex gel filtration, and QAE-Sephadex gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.11 muscle
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.11 0.41
-
 crude extract, at pH 7.8 Klebsiella pneumoniae
4.2.1.11 0.9
-
 crude extract, at pH 6.8 Homo sapiens
4.2.1.11 31.2
-
 after 76.1fold purification, at pH 7.8 Klebsiella pneumoniae
4.2.1.11 75
-
 after 83fold purification, at pH 6.8 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate
-
 Homo sapiens phosphoenolpyruvate + H2O
-
 r
4.2.1.11 2-phospho-D-glycerate
-
 Klebsiella pneumoniae phosphoenolpyruvate + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.11 homodimer 2 * 45000, SDS-PAGE Homo sapiens
4.2.1.11 homodimer 2 * 47000, SDS-PAGE Klebsiella pneumoniae

Synonyms

EC Number Synonyms Comment Organism
4.2.1.11 2-phosphopyruvate hydrolyase
-
 Homo sapiens
4.2.1.11 2-phosphopyruvate hydrolyase
-
 Klebsiella pneumoniae
4.2.1.11 enolase
-
 Homo sapiens
4.2.1.11 enolase
-
 Klebsiella pneumoniae