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Literature summary extracted from

  • Jackson, C.J.; Hadler, K.S.; Carr, P.D.; Oakley, A.J.; Yip, S.; Schenk, G.; Ollis, D.L.
    Malonate-bound structure of the glycerophosphodiesterase from Enterobacter aerogenes (GpdQ) and characterization of the native Fe2+ metal-ion preference (2008), Acta Crystallogr. Sect. F, 64, 681-685.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.46
-
 Klebsiella aerogenes

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.4.46
-
 Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.46 3.5
-
 bis(p-nitrophenyl phosphate) in the presence of 0.1 mM Zn2+ Klebsiella aerogenes
3.1.4.46 7
-
 bis(p-nitrophenyl phosphate) in the presence of 0.1 mM Fe2+ Klebsiella aerogenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.46 Fe2+
-
 Klebsiella aerogenes

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.46 Klebsiella aerogenes Q6XBH1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.46
-
 Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.46 bis(p-nitrophenyl) phosphate + H2O
-
 Klebsiella aerogenes p-nitrophenyl phosphate + p-nitrophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.46 glycerophosphodiesterase
-
 Klebsiella aerogenes

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.4.46 0.176
-
 bis(p-nitrophenyl phosphate) in the presence of 0.1 mM Zn2+ Klebsiella aerogenes
3.1.4.46 2.78
-
 bis(p-nitrophenyl phosphate) in the presence of 0.1 mM Fe2+ Klebsiella aerogenes

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.4.46 0.0498
-
 bis(p-nitrophenyl phosphate) in the presence of 0.1 mM Zn2+ Klebsiella aerogenes
3.1.4.46 0.4
-
 bis(p-nitrophenyl phosphate) in the presence of 0.1 mM Fe2+ Klebsiella aerogenes