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Literature summary extracted from

  • Hetmann, A.; Kowalczyk, S.
    Nucleoside diphosphate kinase isoforms regulated by phytochrome A isolated from oat coleoptiles (2009), Acta Biochim. Pol., 56, 143-153.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.6 cytoplasm more than 80% of total activity is detected in the cytoplasm Avena sativa 5737
-
2.7.4.6 etioplast
-
 Avena sativa 9513
-
2.7.4.6 mitochondrion
-
 Avena sativa 5739
-
2.7.4.6 nucleus
-
 Avena sativa 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.6 17000
-
 4 * 17000, isoforms NDPK In and NDPK IIpn, gel filtration Avena sativa
2.7.4.6 17000 18000 SDS-PAGE Avena sativa
2.7.4.6 63000
-
 NDPK IIpn isoform, gel filtration Avena sativa
2.7.4.6 71000
-
 NDPK In isoform, gel filtration Avena sativa

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.6 Avena sativa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.6 ammonium sulfate precipitation, Bio-Gel hydroxyapatite column chromatography, GTP-agarose column chromatography, and DEAE-Sephacelgel filtration Avena sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.6 coleoptile
-
 Avena sativa
-
2.7.4.6 seedling
-
 Avena sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.6 ATP + NDP
-
 Avena sativa ADP + NTP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.6 homotetramer 4 * 17000, isoforms NDPK In and NDPK IIpn, gel filtration Avena sativa

Synonyms

EC Number Synonyms Comment Organism
2.7.4.6 NDPK IIpn isoform Avena sativa
2.7.4.6 NDPK In isoform Avena sativa
2.7.4.6 nucleoside diphosphate kinase
-
 Avena sativa

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.4.6 ATP
-
 Avena sativa

Expression

EC Number Organism Comment Expression
2.7.4.6 Avena sativa exposure of etiolated oat seedlings to red light causes an increase of the enzyme activity by about 42% in nuclear fraction, 7% in etioplastic and 14% in postetioplastic fraction, one of the nuclear isoforms (In) and one isoform isolated from the postnuclear fraction (IIpn) are activated by red light in the presence of phytochrome A purified from etiolated oat coleoptiles up