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Literature summary extracted from
- Evidences of Hfq associates with tryptophanase and affects extracellular indole levels (2009), Acta Biochim. Biophys. Sin., 41, 709-717.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.99.1 | 0.1732 | - |
L-Trp | in the presence of Hfq protein, in 0.1 M potassium phosphate buffer (pH 7.8), at 37°C | Escherichia coli |  |
| 4.1.99.1 | 0.1901 | - |
L-Trp | in the absence of Hfq protein, in 0.1 M potassium phosphate buffer (pH 7.8), at 37°C | Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.99.1 | 50000 | - |
tryptophanase subunit, SDS-PAGE | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.99.1 | Escherichia coli | - |
strains MG1655, MG1406, and MG1655H | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.1.99.1 | 0.508 | - |
cell extract from strain MG1655H, harvested at OD600 of 0.8, at 37°C | Escherichia coli |
| 4.1.99.1 | 0.747 | - |
cell extract from strain MG1655, harvested at OD600 of 0.4, at 37°C | Escherichia coli |
| 4.1.99.1 | 0.774 | - |
cell extract from strain MG1406, harvested at OD600 of 0.4, at 37°C | Escherichia coli |
| 4.1.99.1 | 0.808 | - |
cell extract from strain MG1655H, harvested at OD600 of 0.4, at 37°C | Escherichia coli |
| 4.1.99.1 | 0.818 | - |
cell extract from strain MG1655, harvested at OD600 of 0.8, at 37°C | Escherichia coli |
| 4.1.99.1 | 1.475 | - |
cell extract from strain MG1406, harvested at OD600 of 0.8, at 37°C | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.99.1 | L-Trp + H2O | - |
Escherichia coli | indole + pyruvate + NH4+ | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.99.1 | Trpase | - |
Escherichia coli |
| 4.1.99.1 | tryptophan indole-lyase | - |
Escherichia coli |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.99.1 | pyridoxal 5'-phosphate | - |
Escherichia coli | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 4.1.99.1 | physiological function | Hfq protein associates with tryptophanase under relatively higher extracellular indole levels, suggesting this is a feedback control of indole production | Escherichia coli |
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