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Literature summary extracted from

  • Calandrelli, V.; Gambacorta, A.; Romano, I.; Carratore, V.; Lama, L.
    A novel thermo-alkali stable catalase-peroxidase from Oceanobacillus oncorhynchi subsp. incaldaniensis: Purification and characterization (2008), World J. Microbiol. Biotechnol., 24, 2269-2275.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.11.1.21 NaCl 76% stimulation of catalase by addition of 10 mM NaCl Oceanobacillus oncorhynchi subsp. incaldanensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.6 3-Amino-1,2,4-triazole 3-amino-1,2,4-triazole at concentrations of 10 mM has no inhibition on the activity Oceanobacillus oncorhynchi
1.11.1.6 Ba2+ partial inhibition at 2 mM Oceanobacillus oncorhynchi
1.11.1.6 Co2+ partial inhibition at 2 mM Oceanobacillus oncorhynchi
1.11.1.6 EDTA 10 mM EDTA reduces activity by 27% Oceanobacillus oncorhynchi
1.11.1.6 H2O2 H2O2 causes 17% inhibition at 80 mM Oceanobacillus oncorhynchi
1.11.1.6 histidine 10 mM histidine reduces activity by 19% Oceanobacillus oncorhynchi
1.11.1.6 Hydroxylamine hydrochloride strong inhibition Oceanobacillus oncorhynchi
1.11.1.6 Sodium azide strong inhibition Oceanobacillus oncorhynchi
1.11.1.21 azide strong inhibition Oceanobacillus oncorhynchi subsp. incaldanensis
1.11.1.21 Ba2+ partial inhibition of catalase activity Oceanobacillus oncorhynchi subsp. incaldanensis
1.11.1.21 Co2+ partial inhibition of catalase activity Oceanobacillus oncorhynchi subsp. incaldanensis
1.11.1.21 cyanide
-
 Oceanobacillus oncorhynchi subsp. incaldanensis
1.11.1.21 EDTA 27% inhibition at 10 mM Oceanobacillus oncorhynchi subsp. incaldanensis
1.11.1.21 H2O2 17% inhibition at 80 mM Oceanobacillus oncorhynchi subsp. incaldanensis
1.11.1.21 histidine 19% inhibition at 10 mM Oceanobacillus oncorhynchi subsp. incaldanensis
1.11.1.21 hydroxylamine strong inhibition Oceanobacillus oncorhynchi subsp. incaldanensis
1.11.1.21 KCl 50% inhibition at 20 mM Oceanobacillus oncorhynchi subsp. incaldanensis
1.11.1.21 MgCl2 50% inhibition at 10 mM Oceanobacillus oncorhynchi subsp. incaldanensis
1.11.1.21 additional information the enzyme is insensitive to 10 mM 3-amino-1,2,4-triazole Oceanobacillus oncorhynchi subsp. incaldanensis
1.11.1.21 NaCl 50% inhibition at 5 mM Oceanobacillus oncorhynchi subsp. incaldanensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.6 24
-
 H2O2 in 20 mM Tris-HCl buffer (pH 9.0), at 30°C Oceanobacillus oncorhynchi
1.11.1.21 24
-
 H2O2 catalase activity, in 20 mM Tris-HCl buffer (pH 9.0), at 30°C Oceanobacillus oncorhynchi subsp. incaldanensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.21 cytosol
-
 Oceanobacillus oncorhynchi subsp. incaldanensis 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.6 Fe2+ contains 1 atom Fe2+ per homodimer Oceanobacillus oncorhynchi
1.11.1.6 K+ stimulatory effect at 2 mM Oceanobacillus oncorhynchi
1.11.1.6 Mg2+ stimulatory effect at 2 mM Oceanobacillus oncorhynchi
1.11.1.6 Mn2+ stimulatory effect at 2 mM Oceanobacillus oncorhynchi
1.11.1.6 Na+ stimulatory effect at 2 mM Oceanobacillus oncorhynchi
1.11.1.6 NaCl there is a stimulation of catalase by addition of NaCl 10 mM (176%) Oceanobacillus oncorhynchi
1.11.1.21 Fe2+ iron is present at 1 atom per homodimer Oceanobacillus oncorhynchi subsp. incaldanensis
1.11.1.21 K+ 2 mM K+ have a stimulatory effect on catalase activity Oceanobacillus oncorhynchi subsp. incaldanensis
1.11.1.21 Mg2+ 2 mM Mg2+ have a stimulatory effect on catalase activity Oceanobacillus oncorhynchi subsp. incaldanensis
1.11.1.21 Mn2+ 2 mM Mn2+ have a stimulatory effect on catalase activity Oceanobacillus oncorhynchi subsp. incaldanensis
1.11.1.21 Na+ 2 mM Na+ have a stimulatory effect on catalase activity Oceanobacillus oncorhynchi subsp. incaldanensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.6 72000
-
 4 * 72000, SDS-PAGE Oceanobacillus oncorhynchi
1.11.1.6 280000
-
 gel filtration Oceanobacillus oncorhynchi
1.11.1.21 72000
-
 4 * 72000, SDS-PAGE Oceanobacillus oncorhynchi subsp. incaldanensis
1.11.1.21 280000
-
 gel filtration Oceanobacillus oncorhynchi subsp. incaldanensis

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.6 Oceanobacillus oncorhynchi
-
 subspecies Oceanobacillus oncorhynchi incaldaniensis, strain 20AG
-
1.11.1.21 Oceanobacillus oncorhynchi subsp. incaldanensis
-
-
-
1.11.1.21 Oceanobacillus oncorhynchi subsp. incaldanensis 20AG
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.6 Q-Sepharose column chromatography, Superdex 200 gel filtration, and Mono Q column chromatography Oceanobacillus oncorhynchi
1.11.1.21 Q-Sepharose column chromatography, Superdex 200 gel filtration, and Mono Q column chromatography Oceanobacillus oncorhynchi subsp. incaldanensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.6 316
-
 crude extract, at 30°C Oceanobacillus oncorhynchi
1.11.1.6 35000
-
 after 110.7fold purification, at 30°C Oceanobacillus oncorhynchi
1.11.1.21 168
-
 unpurified cytosolic fraction, peroxidase activity, in 50 mM Tris-HCl buffer (pH 8.5), at 30°C Oceanobacillus oncorhynchi subsp. incaldanensis
1.11.1.21 316
-
 unpurified cytosolic fraction, catalase activity, in 20 mM Tris-HCl buffer (pH 9.0), at 30°C Oceanobacillus oncorhynchi subsp. incaldanensis
1.11.1.21 7512
-
 after 44.7fold purification, peroxidase activity, in 50 mM Tris-HCl buffer (pH 8.5), at 30°C Oceanobacillus oncorhynchi subsp. incaldanensis
1.11.1.21 35000
-
 after 110.7fold purification, catalase activity, in 20 mM Tris-HCl buffer (pH 9.0), at 30°C Oceanobacillus oncorhynchi subsp. incaldanensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.6 H2O2
-
 Oceanobacillus oncorhynchi O2 + H2O
-
 ?
1.11.1.21 4-aminoantipyrine + H2O2
-
 Oceanobacillus oncorhynchi subsp. incaldanensis oxidized 4-aminoantipyrine + H2O
-
 ?
1.11.1.21 4-aminoantipyrine + H2O2
-
 Oceanobacillus oncorhynchi subsp. incaldanensis 20AG oxidized 4-aminoantipyrine + H2O
-
 ?
1.11.1.21 H2O2
-
 Oceanobacillus oncorhynchi subsp. incaldanensis O2 + H2O
-
 ?
1.11.1.21 H2O2
-
 Oceanobacillus oncorhynchi subsp. incaldanensis 20AG O2 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.6 homotetramer 4 * 72000, SDS-PAGE Oceanobacillus oncorhynchi
1.11.1.21 homotetramer 4 * 72000, SDS-PAGE Oceanobacillus oncorhynchi subsp. incaldanensis

Synonyms

EC Number Synonyms Comment Organism
1.11.1.6 catalase-peroxidase
-
 Oceanobacillus oncorhynchi
1.11.1.6 H2O2:H2O2 oxidoreductase
-
 Oceanobacillus oncorhynchi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.6 40
-
-
 Oceanobacillus oncorhynchi
1.11.1.21 40
-
 for catalase activity Oceanobacillus oncorhynchi subsp. incaldanensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.11.1.6 30 60
-
 Oceanobacillus oncorhynchi
1.11.1.21 30 60 for catalase activity Oceanobacillus oncorhynchi subsp. incaldanensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.6 40 60 the enzyme is stable for 24 h at 40°C with a half-life of 4 h 60°C, at 50°C the half life is about 18 h Oceanobacillus oncorhynchi
1.11.1.21 40 60 the catalatic activity of the enzyme is stable for 24 h at 40°C. At 50°C the half life is about 18 h, while at 60°C over the first 4 h of incubation 50% of activity is observed Oceanobacillus oncorhynchi subsp. incaldanensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.6 9
-
-
 Oceanobacillus oncorhynchi
1.11.1.21 9
-
 for catalase activity Oceanobacillus oncorhynchi subsp. incaldanensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.6 5 10
-
 Oceanobacillus oncorhynchi
1.11.1.21 5 10 for catalase activity Oceanobacillus oncorhynchi subsp. incaldanensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.6 5 10 the enzyme is stable in the pH range of 5.0 to 10.0 after 1 h of treatment at 40°C Oceanobacillus oncorhynchi
1.11.1.21 5 10 the catalatic activity of the enzyme is stable in the pH range of 5.0 to 10.0 after 1 h of treatment at 40°C Oceanobacillus oncorhynchi subsp. incaldanensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.6 heme contains 1 heme per homodimer Oceanobacillus oncorhynchi
1.11.1.21 heme the enzyme contains 1 heme per homodimer Oceanobacillus oncorhynchi subsp. incaldanensis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.11.1.21 5
-
 catalase activity, in 20 mM Tris-HCl buffer (pH 9.0), at 30°C Oceanobacillus oncorhynchi subsp. incaldanensis NaCl
1.11.1.21 10
-
 catalase activity, in 20 mM Tris-HCl buffer (pH 9.0), at 30°C Oceanobacillus oncorhynchi subsp. incaldanensis MgCl2
1.11.1.21 20
-
 catalase activity, in 20 mM Tris-HCl buffer (pH 9.0), at 30°C Oceanobacillus oncorhynchi subsp. incaldanensis KCl