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Literature summary extracted from

  • Harbeck, M.C.; Cheung, A.Y.; Suttie, J.W.
    Vitamin K-dependent carboxylase: partial purification of the enzyme by antibody affinity techniques (1989), Thromb. Res., 56, 317-323.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.90 2-chloro-3-phytyl-1,4-naphthoquinone
-
 Bos taurus
4.1.1.90 tetrachloropyridin
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.90 microsome from a dicoumarol-treated cow Bos taurus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.90 L-glutamate + CO2 + O2 + vitamin K hydroquinone Bos taurus
-
 gamma-carboxy L-glutamate + vitamin K epoxide + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.90 Bos taurus Q07175
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.90 partial purification of the enzyme by antibody affinity techniques. Purified 500fold by adsorption to an antiprothrombin column and elution with a dodeca peptide which competes with a prothrombin precursor enzyme recognition site. The purified enzyme is devoid of bound precursors, and has the same ratio of vitamin K epoxidase activity to carboxylase activity as the crude microsomal preparation Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.90 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.90 additional information
-
 propeptide eluate of gel: 608 U/mg, carboxylase units are expressed as 100000 dpm of 14CO2 fixed in the standard assay Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.90 L-glutamate + CO2 + O2 + vitamin K hydroquinone
-
 Bos taurus gamma-carboxy L-glutamate + vitamin K epoxide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.90 glutamate carboxylase
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.90 vitamin K
-
 Bos taurus