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Literature summary extracted from

  • Dimastrogiovanni, D.; Anselmi, M.; Miele, A.E.; Boumis, G.; Petersson, L.; Angelucci, F.; Nola, A.D.; Brunori, M.; Bellelli, A.
    Combining crystallography and molecular dynamics: The case of Schistosoma mansoni phospholipid glutathione peroxidase (2009), Proteins, 78, 259-270.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.9 expressed in Escherichia coli BL21(DE3) cells Schistosoma mansoni
1.11.1.12 the codon optimized gene is cloned into the vector pGex4T-1 for expression in Escherichia coli BL21DE3 cells Schistosoma mansoni

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.9 sitting drop vapor diffusion method, using 0.2 M LiSO4,0.2 M sodium acetate, 24% (w/v) PEG 8000, pH 4.5 for mutant enzyme U43C and 0.2 M NaH2PO4, 0.1 M MES, 32% (w/v) PEG-MME 5000, pH 6.0 for mutant enzyme U43S Schistosoma mansoni
1.11.1.12 the crystal structures of the Sec43Cys and Sec43Ser mutants are solved at resolutions of 1.0 A and 1.7 A, respectively Schistosoma mansoni

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.9 U43C inactive Schistosoma mansoni
1.11.1.9 U43S inactive Schistosoma mansoni
1.11.1.12 Sec43Cys mutation of the selenium cysteine in the active site Schistosoma mansoni
1.11.1.12 Sec43Ser mutation of the selenium cysteine in the active site Schistosoma mansoni

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.9 selenium Gpx is a selenium-containing enzyme Schistosoma mansoni
1.11.1.12 selenium
-
 Schistosoma mansoni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.9 19400
-
 1 * 19400, estimated from SDS-PAGE Schistosoma mansoni
1.11.1.12 19400
-
 determined by gel filtration Schistosoma mansoni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.9 H2O2 + GSH Schistosoma mansoni
-
 H2O + GSSG
-
 ?
1.11.1.9 tert-butyl hydroperoxide + 2 GSH Schistosoma mansoni
-
 tert-butyl alcohol + GSSG + H2O
-
 ?
1.11.1.12 glutathione + a lipid hydroperoxide Schistosoma mansoni
-
 glutathione disulfide + lipid + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.9 Schistosoma mansoni Q00277
-
-
1.11.1.12 Schistosoma mansoni
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.9 glutathione-Sepharose column chromatography and HiTrap SP FF column chromatography Schistosoma mansoni
1.11.1.12 Sec43Cys is purified on a GSH-Sepharose column, Sec43Ser is purified by ionic exchange chromatography, the GST-tags are cleaved with bovine thrombin Schistosoma mansoni

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.9 integument
-
 Schistosoma mansoni
-
1.11.1.12 integument
-
 Schistosoma mansoni
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.9 H2O2 + GSH
-
 Schistosoma mansoni H2O + GSSG
-
 ?
1.11.1.9 tert-butyl hydroperoxide + 2 GSH
-
 Schistosoma mansoni tert-butyl alcohol + GSSG + H2O
-
 ?
1.11.1.12 glutathione + a lipid hydroperoxide
-
 Schistosoma mansoni glutathione disulfide + lipid + H2O
-
 ?
1.11.1.12 glutathione + H2O2 activity assay Schistosoma mansoni ?
-
 ?
1.11.1.12 glutathione + tert-butyl-hydroperoxide activity assay Schistosoma mansoni ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.9 monomer 1 * 19400, estimated from SDS-PAGE Schistosoma mansoni
1.11.1.12 monomer
-
 Schistosoma mansoni

Synonyms

EC Number Synonyms Comment Organism
1.11.1.9 GPx4
-
 Schistosoma mansoni
1.11.1.9 phospholipid glutathione peroxidase
-
 Schistosoma mansoni
1.11.1.9 phospholipid hydroperoxide Gpx
-
 Schistosoma mansoni
1.11.1.12 class 4 GPx
-
 Schistosoma mansoni
1.11.1.12 phospholipid glutathione peroxidase
-
 Schistosoma mansoni
1.11.1.12 seleno GPx
-
 Schistosoma mansoni

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.12 25
-
 activity assay Schistosoma mansoni

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.12 7.4
-
 activity assay Schistosoma mansoni

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.12 glutathione
-
 Schistosoma mansoni