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Literature summary extracted from

  • Kim, H.Y.; Zhang, Y.; Lee, B.C.; Kim, J.R.; Gladyshev, V.N.
    The selenoproteome of Clostridium sp. OhILAs: characterization of anaerobic bacterial selenoprotein methionine sulfoxide reductase A (2009), Proteins, 74, 1008-1017.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.11 expressed in Escherichia coli BL21(DE3) cells Clostridium sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.8.4.11 additional information the cysteine mutant form U16C exhibits at least a 20fold lower activity than the selenocysteine-containing wild type MsrA enzyme Clostridium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.4.11 4
-
 dabsylated L-methionine (S)-sulfoxide purified cysteine mutant form U16C, using dithiothreitol as cosubstrate, in 50 mM sodium phosphate, pH 7.5, at 37°C Clostridium sp.
1.8.4.11 10.2
-
 dabsylated L-methionine (S)-sulfoxide wild type enzyme, using dithiothreitol as cosubstrate, in 50 mM sodium phosphate, pH 7.5, at 37°C Clostridium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.4.11 selenium selenoprotein, has a single selenocysteine residue at the catalytic site, the selenocysteine-containing MsrA exhibits at least a 20fold higher activity than its cysteine mutant form U16C, indicating a critical role of selenocysteine in the catalytic activity of the enzyme Clostridium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.11 Clostridium sp.
-
 also known as Alkaliphilus oremlandii
-
1.8.4.11 Clostridium sp. OhILAs
-
 also known as Alkaliphilus oremlandii
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.11 Talon-metal affinity resin chromatography Clostridium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.4.11 0.014
-
 purified cysteine mutant form U16C, using human thioredoxin as cosubstrate, in 50 mM sodium phosphate, pH 7.5, at 37°C Clostridium sp.
1.8.4.11 0.023
-
 purified cysteine mutant form U16C, using thioredoxin from Escherichia coli as cosubstrate, in 50 mM sodium phosphate, pH 7.5, at 37°C Clostridium sp.
1.8.4.11 0.034
-
 purified wild type enzyme, using human thioredoxin as cosubstrate, in 50 mM sodium phosphate, pH 7.5, at 37°C Clostridium sp.
1.8.4.11 0.07
-
 purified wild type enzyme, using Escherichia coli thioredoxin as cosubstrate, in 50 mM sodium phosphate, pH 7.5, at 37°C Clostridium sp.
1.8.4.11 0.464
-
 purified cysteine mutant form U16C, using dithiothreitol as cosubstrate, in 50 mM sodium phosphate, pH 7.5, at 37°C Clostridium sp.
1.8.4.11 4.185
-
 purified wild type enzyme, using dithiothreitol as cosubstrate, in 50 mM sodium phosphate, pH 7.5, at 37°C Clostridium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.11 dabsyl-L-methionine (S)-sulfoxide + dithiothreitol
-
 Clostridium sp. dabsyl-L-methionine + dithiothreitol disulfide
-
 ?
1.8.4.11 dabsyl-L-methionine (S)-sulfoxide + dithiothreitol
-
 Clostridium sp. OhILAs dabsyl-L-methionine + dithiothreitol disulfide
-
 ?
1.8.4.11 dabsyl-L-methionine (S)-sulfoxide + thioredoxin
-
 Clostridium sp. dabsyl-L-methionine + thioredoxin disulfide
-
 ?
1.8.4.11 dabsyl-L-methionine (S)-sulfoxide + thioredoxin
-
 Clostridium sp. OhILAs dabsyl-L-methionine + thioredoxin disulfide
-
 ?
1.8.4.11 dabsylated L-methionine (S)-sulfoxide + thioredoxin
-
 Clostridium sp. dabsylated L-methionine + thioredoxin disulfide + H2O
-
 r
1.8.4.11 dabsylated L-methionine (S)-sulfoxide + thioredoxin
-
 Clostridium sp. OhILAs dabsylated L-methionine + thioredoxin disulfide + H2O
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.8.4.11 methionine sulfoxide reductase A
-
 Clostridium sp.
1.8.4.11 MsrA
-
 Clostridium sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.4.11 3.8
-
 dabsylated L-methionine (S)-sulfoxide purified cysteine mutant form U16C, using dithiothreitol as cosubstrate, in 50 mM sodium phosphate, pH 7.5, at 37°C Clostridium sp.
1.8.4.11 84
-
 dabsylated L-methionine (S)-sulfoxide wild type enzyme, using dithiothreitol as cosubstrate, in 50 mM sodium phosphate, pH 7.5, at 37°C Clostridium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.4.11 dithiothreitol
-
 Clostridium sp.
1.8.4.11 thioredoxin Clostridium MsrA is inefficiently reducible by thioredoxin Clostridium sp.