EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.21.1 | 2-nitro-5-thiosulfobenzoic acid | the presence of Ca2+ or Mg2+ at pH 7.5 results in 80% inactivation without fragmentation of the enzyme. In the absence of metal ions it retains 80% of its activity. It binds DNase I through covalent modification, since dialysis and gel filtration can not reverse the inactivation reaction. After dilution into an acid buffer of pH 4.7, the inactivated enzyme regains about 40% of its initial activity. The inhibitor fails to inactivate other enzymes, suggesting that the inhibition is unique to DNase I | Bos taurus | |
3.1.21.1 | 2-nitro-5-thiosulfobenzoic acid | is a novel inhibitor specific for DNase I | Rattus norvegicus | |
3.1.21.1 | additional information | DTNB or Na2SO3 alone do not inactivate DNase I, even in the presence of divalent cations | Bos taurus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.21.1 | Ca2+ | in the presence of Ca2+, native (0.2 U) and inactivated DNase I of 5fold greater enzyme units applied (1.0 U) hydrolyze the Mg2+-DNA substrate forming some linear duplex DNA in addition to the relaxed open-circular DNA, indicating double scission, while 0.2 U of 2-nitro-5-thiosulfobenzoic acid-treated DNase I can only cleave the Mg2+-DNA substrate in a single nicking mode | Bos taurus | |
3.1.21.1 | Mg2+ | in the absence of Ca2+, only native DNase I cleaves the Mg2+-DNA substrate in a single nicking mode with the formation of only the relaxed open-circular DNA, while the 2-nitro-5-thiosulfobenzoic acid-treated DNase I fails to cleave the plasmid DNA substrate | Bos taurus | |
3.1.21.1 | Mn2+ | in the presence of Mn2+, native and 2-nitro-5-thiosulfobenzoic acid-treated DNase I of two different enzyme units used are all able to hydrolyze the plasmid DNA substrate in a double scission mode | Bos taurus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.21.1 | Bos taurus | - |
- |
- |
3.1.21.1 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.21.1 | - |
Rattus norvegicus |
3.1.21.1 | - |
Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.21.1 | pancreas | - |
Bos taurus | - |
3.1.21.1 | parotid gland | - |
Rattus norvegicus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.21.1 | plasmid DNA + H2O | depending on metal ions, duplex DNA is hydrolyzed by DNase I in a single or double scission mode | Bos taurus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.21.1 | DNase I | - |
Rattus norvegicus |
3.1.21.1 | DNase I | - |
Bos taurus |