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Literature summary extracted from

  • Harnpicharnchai, P.; Champreda, V.; Sornlake, W.; Eurwilaichitr, L.
    A thermotolerant beta-glucosidase isolated from an endophytic fungi, Periconia sp., with a possible use for biomass conversion to sugars (2008), Protein Expr. Purif., 67, 61-69.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.21 synthesis enzyme is able to hydrolyze rice straw into simple sugars Periconia sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21
-
 Periconia sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 Ba2+ 1 mM, 58% residual activity Periconia sp.
3.2.1.21 Cu2+ 1 mM, 5.1% residual activity Periconia sp.
3.2.1.21 Fe2+ 1 mM, 30% residual activity Periconia sp.
3.2.1.21 Zn2+ 1 mM, 51% residual activity Periconia sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.04
-
 4-methylumbelliferyl beta-D-cellobioside pH 5.0, 40°C Periconia sp.
3.2.1.21 0.19
-
 4-nitrophenyl-beta-D-glucopyranoside pH 5.0, 40°C Periconia sp.
3.2.1.21 0.5
-
 cellobiose pH 5.0, 40°C Periconia sp.
3.2.1.21 4.73
-
 4-nitrophenyl-alpha-D-glucopyranoside pH 5.0, 40°C Periconia sp.
3.2.1.21 11.57
-
 4-nitrophenyl-beta-D-xylopyranoside pH 5.0, 40°C Periconia sp.
3.2.1.21 22.5
-
 4-nitrophenyl-beta-D-fucopyranoside pH 5.0, 40°C Periconia sp.

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.21 2-propanol at 10%, 107%, at 50%, 0.2% of initial activity, respectively Periconia sp.
3.2.1.21 Ethanol at 10%, 127%, at 50%, 0.6% of initial activity, respectively Periconia sp.
3.2.1.21 Methanol at 10%, 121%, at 50%, 1.3% of initial activity, respectively Periconia sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Periconia sp. A9UIG0
-
-

Storage Stability

EC Number Storage Stability Organism
3.2.1.21 4°C, MOPS buffer pH 6.0, stable for at least 6 months Periconia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-methylumbelliferyl beta-D-cellobioside + 2 H2O
-
 Periconia sp. 4-methylumbelliferol + 2 D-glucose
-
 ?
3.2.1.21 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Periconia sp. 4-nitrophenol + D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-arabinopyranoside + H2O
-
 Periconia sp. 4-nitrophenol + D-arabinose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-fucopyranoside + H2O
-
 Periconia sp. 4-nitrophenol + D-fucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Periconia sp. 4-nitrophenol + D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Periconia sp. 4-nitrophenol + D-xylose
-
 ?
3.2.1.21 4-nitrophenyl-alpha-D-xylopyranoside + H2O
-
 Periconia sp. 4-nitrophenol + D-xylose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Periconia sp. 2 D-glucose
-
 ?
3.2.1.21 additional information enzyme is able to hydrolyze rice straw into simple sugars Periconia sp. ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 70
-
-
 Periconia sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 additional information
-
 thermostability is improved in presence of cellobiose, glucose, or sucrose Periconia sp.
3.2.1.21 70
-
 1.5 h, 60% residual activity Periconia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 5
-
 and 6 Periconia sp.
3.2.1.21 6
-
 and 5 Periconia sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.21 8
-
 and above, 2 h, no loss of activity Periconia sp.