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Literature summary extracted from

  • Emerson, J.P.; Kovaleva, E.G.; Farquhar, E.R.; Lipscomb, J.D.; Que, L.
    Swapping metals in Fe- and Mn-dependent dioxygenases: evidence for oxygen activation without a change in metal redox state (2008), Proc. Natl. Acad. Sci. USA, 105, 7347-7352.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.15 Sodium ascorbate 1 mM, revers purification inactivation of Fe-MndD, about 30-35% higher catalytic activity than the purified enzyme Arthrobacter globiformis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.15 expression in Escherichia coli BL21 Brevibacterium fuscum
1.13.11.15 expression in Escherichia coli DH5alpha Arthrobacter globiformis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.15 X-ray Arthrobacter globiformis
1.13.11.15 X-ray Brevibacterium fuscum

General Stability

EC Number General Stability Organism
1.13.11.15 Fe-HPCD stable in air for at least several days Brevibacterium fuscum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.15 air initially high activity of Fe-MndD, becomes inactivtated within one h Arthrobacter globiformis
1.13.11.15 ferricyanide 1 mM, inactivates Fe-MndD Arthrobacter globiformis
1.13.11.15 ferricyanide 1 mM, inactivates Fe-HPCD Brevibacterium fuscum
1.13.11.15 H2O2 1 mM, inactivates Fe-MndD Arthrobacter globiformis
1.13.11.15 H2O2 1 mM, inactivates Fe-HPCD Brevibacterium fuscum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.15 0.014
-
 3,4-Dihydroxyphenylacetate Mn-MndD Arthrobacter globiformis
1.13.11.15 0.015
-
 3,4-Dihydroxyphenylacetate Fe-MndD Arthrobacter globiformis
1.13.11.15 0.031
-
 3,4-Dihydroxyphenylacetate Fe-HPCD Brevibacterium fuscum
1.13.11.15 0.035
-
 3,4-Dihydroxyphenylacetate Mn-HPCD Brevibacterium fuscum
1.13.11.15 0.037
-
 O2 Fe-MndD Arthrobacter globiformis
1.13.11.15 0.05
-
 O2 Mn-HPCD Brevibacterium fuscum
1.13.11.15 0.06
-
 O2 Fe-HPCD Brevibacterium fuscum
1.13.11.15 0.062
-
 O2 Mn-MndD Arthrobacter globiformis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.15 Fe2+ incorporated in Fe-HPCD Brevibacterium fuscum
1.13.11.15 Fe2+ nonphysiological metal, incorporated in Fe-MndD Arthrobacter globiformis
1.13.11.15 Fe3+ nonphysiological metal, incorporated in Fe-MndD Arthrobacter globiformis
1.13.11.15 Mn2+ incorporated in Mn-MndD Arthrobacter globiformis
1.13.11.15 Mn2+ nonphysiological metal, incorporated in Mn-HPCD Brevibacterium fuscum

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.15 Arthrobacter globiformis
-
-
-
1.13.11.15 Brevibacterium fuscum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.15
-
 Arthrobacter globiformis
1.13.11.15
-
 Brevibacterium fuscum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.15 3,4-dihydroxyphenylacetate + O2 1 mM, assay at pH 7.8, 23°C Arthrobacter globiformis ?
-
 ?
1.13.11.15 3,4-dihydroxyphenylacetate + O2 1 mM, assay at pH 7.8, 23°C Brevibacterium fuscum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.15 Fe-HPCD Fe(II) incorporated Brevibacterium fuscum
1.13.11.15 Fe-MndD Fe incorporated Arthrobacter globiformis
1.13.11.15 homoprotocatechuate 2,3-dioxygenase
-
 Arthrobacter globiformis
1.13.11.15 homoprotocatechuate 2,3-dioxygenase
-
 Brevibacterium fuscum
1.13.11.15 Mn-HPCD Mn(II) incorporated Brevibacterium fuscum
1.13.11.15 Mn-MndD Mn(II) incorporated Arthrobacter globiformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.15 23
-
 assay at Arthrobacter globiformis
1.13.11.15 23
-
 assay at Brevibacterium fuscum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.15 7.8
-
 assay at Arthrobacter globiformis
1.13.11.15 7.8
-
 assay at Brevibacterium fuscum