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Literature summary extracted from

  • Leferink, N.G.; van Duijn, E.; Barendregt, A.; Heck, A.J.; van Berkel, W.J.
    Galactonolactone dehydrogenase requires a redox-sensitive thiol for optimal production of vitamin C (2009), Plant Physiol., 150, 596-605.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.2.3 into a pET-His6 vector for expression in Escherichia coli BL21DE3 cells Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.3.2.3 C340A mutant, insensitive toward thiol oxidation, exhibits poor affinity for L-galactono-1,4-lactone Arabidopsis thaliana
1.3.2.3 C340S mutant, insensitive toward thiol oxidation, exhibits poor affinity for L-galactono-1,4-lactone Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.2.3 5,5'-dithiobis(2-nitrobenzoic acid) thiol-modifying agent Arabidopsis thaliana
1.3.2.3 H2O2 GALDH is inactivated by hydrogen peroxide due to selective oxidation of cysteine 340, located in the cap domain Arabidopsis thaliana
1.3.2.3 N-ethylmaleimide thiol-modifying agent Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.2.3 0.17
-
 L-galactono-1,4-lactone wild-type enzyme Arabidopsis thaliana
1.3.2.3 5
-
 L-galactono-1,4-lactone mutant C340S Arabidopsis thaliana
1.3.2.3 9
-
 L-galactono-1,4-lactone mutant C340A Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.2.3 mitochondrion
-
 Arabidopsis thaliana 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.2.3 L-galactono-1,4-lactone + ferricytochrome c Arabidopsis thaliana
-
 L-ascorbate + ferrocytochrome c + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.2.3 Arabidopsis thaliana Q9SU56
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.2.3
-
 Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.2.3 L-galactono-1,4-lactone + ferricytochrome c
-
 Arabidopsis thaliana L-ascorbate + ferrocytochrome c + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.2.3 galactonolactone dehydrogenase
-
 Arabidopsis thaliana
1.3.2.3 GALDH
-
 Arabidopsis thaliana
1.3.2.3 L-galactono-gamma-lactone dehydrogenase
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.2.3 59
-
 L-galactono-1,4-lactone mutant C340S Arabidopsis thaliana
1.3.2.3 79
-
 L-galactono-1,4-lactone mutant C340A Arabidopsis thaliana
1.3.2.3 134
-
 L-galactono-1,4-lactone wild-type enzyme Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.2.3 FAD
-
 Arabidopsis thaliana