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Literature summary extracted from

  • Leyva, L.A.; Bashan, Y.
    Activity of two catabolic enzymes of the phosphogluconate pathway in mesquite roots inoculated with Azospirillum brasilense Cd (2008), Plant Physiol. Biochem., 46, 898-904.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.44 additional information external gluconate has no effect on the activity of 6-phosphogluconate dehydrogenase Azospirillum brasilense
2.7.1.12 gluconic acid gluconokinase is induced upon exposure to gluconic acid Azospirillum brasilense

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.44 MgCl2 10 mM are included in assay medium Azospirillum brasilense
2.7.1.12 MgCl2 10 mM are included in assay medium Azospirillum brasilense

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+ Azospirillum brasilense
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?
1.1.1.44 6-phospho-D-gluconate + NADP+ Azospirillum brasilense Cd
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?
2.7.1.12 D-gluconate + ATP Azospirillum brasilense
-
 6-phospho-D-gluconate + ADP
-
 ?
2.7.1.12 D-gluconate + ATP Azospirillum brasilense Cd
-
 6-phospho-D-gluconate + ADP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.44 Azospirillum brasilense
-
-
-
2.7.1.12 Azospirillum brasilense
-
 Cd
-
2.7.1.12 Azospirillum brasilense Cd
-
 Cd
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.44 additional information
-
 6-phosphogluconate dehydrogenase activity in not enhanced in inoculated mesquite plants incubated for 15 or 23 days Azospirillum brasilense
2.7.1.12 additional information
-
 at six concentrations (4.6-90.6 mM) of gluconate added to culture medium for Azospirillum brasilense Cd grown in TYG medium, activity of gluconokinase activity is positively affected Azospirillum brasilense
2.7.1.12 additional information
-
 gluconokinase activity significantly increases in inoculated mesquite plants incubated for 23 days, inoculation is not enhanced after 15 days of incubation Azospirillum brasilense
2.7.1.12 additional information
-
 while increased gluconokinase activity results only in inoculated treatments, older non-inoculated seedlings maintained the same activity as younger seedlings and the sum of activities of the roots and the negative control is still lower than activity in inoculated plants Azospirillum brasilense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Azospirillum brasilense D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Azospirillum brasilense Cd D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?
2.7.1.12 D-gluconate + ATP
-
 Azospirillum brasilense 6-phospho-D-gluconate + ADP
-
 ?
2.7.1.12 D-gluconate + ATP
-
 Azospirillum brasilense Cd 6-phospho-D-gluconate + ADP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.44 6-phosphogluconate dehydrogenase
-
 Azospirillum brasilense
2.7.1.12 gluconokinase
-
 Azospirillum brasilense

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.44 37
-
 assay at Azospirillum brasilense
2.7.1.12 37
-
 assay at Azospirillum brasilense

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.44 7.6
-
 assay at Azospirillum brasilense
2.7.1.12 7.6
-
 assay at Azospirillum brasilense

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.44 NADP+
-
 Azospirillum brasilense
2.7.1.12 ATP
-
 Azospirillum brasilense

Expression

EC Number Organism Comment Expression
2.7.1.12 Azospirillum brasilense glucokinase is induced upon exposure to gluconic acid up

General Information

EC Number General Information Comment Organism
1.1.1.44 metabolism phosphogluconate pathway, bacterial 6-phosphogluconate dehydrogenase is a constitutive enzyme, bacterial enzyme is active in young, non-inoculated mesquite seedlings growing under hydroponic conditions, external gluconate has no effect on the activity of 6-phosphogluconate dehydrogenase Azospirillum brasilense
2.7.1.12 metabolism phosphogluconate pathway, bacterial enzyme is active in young, non-inoculated mesquite seedlings growing under hydroponic conditions Azospirillum brasilense
2.7.1.12 physiological function when azospirillum brasilense Cd bacteria are inoculated on the root system, the roots exhibit much higher activity of gluconokinase Azospirillum brasilense