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Literature summary extracted from

  • Luo, T.; Deng, W.; Zeng, J.; Zhang, F.
    Cloning and characterization of a stearoyl-acyl carrier protein desaturase gene from Cinnamomum longepaniculatum (2009), Plant Mol. Biol. Rep., 27, 13-19.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.2 into the vector pQE-30 for expression in Escherichia coli M15 cells Cinnamomum longipaniculatum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.2 plastid stroma
-
 Cinnamomum longipaniculatum 9532
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.19.2 41800
-
 theoretical Cinnamomum longipaniculatum
1.14.19.2 43500
-
 recombinant 6 x His-SAD, determined by SDS-PAGE Cinnamomum longipaniculatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.2 stearoyl-[acyl-carrier-protein] + reduced acceptor + O2 Cinnamomum longipaniculatum
-
 oleoyl-[acyl-carrier-protein] + acceptor + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.2 Cinnamomum longipaniculatum A8D2K7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.19.2 by gel filtration Cinnamomum longipaniculatum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.2 flower
-
 Cinnamomum longipaniculatum
-
1.14.19.2 leaf
-
 Cinnamomum longipaniculatum
-
1.14.19.2 seed
-
 Cinnamomum longipaniculatum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.19.2 0.0000016
-
 activity in extracts from Escherichia coli M15 transformed with pQESAD, uninduced, with reduced spinach ferredoxin Cinnamomum longipaniculatum
1.14.19.2 0.000002
-
 activity in extracts from Escherichia coli M15 transformed with pQESAD, uninduced, without reduced spinach ferredoxin Cinnamomum longipaniculatum
1.14.19.2 0.0000075
-
 activity in extracts from Escherichia coli M15 transformed with pQESAD, induced, with reduced spinach ferredoxin Cinnamomum longipaniculatum
1.14.19.2 0.000079
-
 activity in extracts from Escherichia coli M15 transformed with pQESAD, induced, without reduced spinach ferredoxin Cinnamomum longipaniculatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.2 stearoyl-[acyl-carrier-protein] + reduced acceptor + O2
-
 Cinnamomum longipaniculatum oleoyl-[acyl-carrier-protein] + acceptor + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.2 CSAD
-
 Cinnamomum longipaniculatum
1.14.19.2 SAD
-
 Cinnamomum longipaniculatum
1.14.19.2 stearoyl-acyl carrier protein desaturase
-
 Cinnamomum longipaniculatum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.19.2 23
-
 activity assay Cinnamomum longipaniculatum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.19.2 6
-
 activity assay Cinnamomum longipaniculatum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.19.2 Cinnamomum longipaniculatum theoretical
-
 5.73