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Literature summary extracted from

  • Pata, M.O.; Wu, B.X.; Bielawski, J.; Xiong, T.C.; Hannun, Y.A.; Ng, C.K.
    Molecular cloning and characterization of OsCDase, a ceramidase enzyme from rice (2008), Plant J., 55, 1000-1009.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.23 into the yeast expression vector pYES2/CT Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.23 0.03934
-
 D-erythro-C12-4-nitrobenzo-2-oxa-1,3-diazole-ceramide apparent KM Oryza sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.23 endoplasmic reticulum
-
 Oryza sativa 5783
-
3.5.1.23 Golgi apparatus
-
 Oryza sativa 5794
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.23 Ca2+ enhances activity Oryza sativa
3.5.1.23 Fe2+ inhibits activity Oryza sativa
3.5.1.23 Mg2+ enhances activity Oryza sativa
3.5.1.23 Mn2+ enhances activity Oryza sativa
3.5.1.23 Zn2+ enhances activity Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.23 87000
-
 determined by SDS-PAGE and Western blot analysis, signal between 75 and 100 kDa Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.23 ceramide + H2O Oryza sativa
-
 sphingosine + fatty acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.23 Oryza sativa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.23 ceramide + H2O
-
 Oryza sativa sphingosine + fatty acid
-
 ?
3.5.1.23 D-erythro-C12-4-nitrobenzo-2-oxa-1,3-diazole-ceramide + H2O substrate activity assay Oryza sativa D-erythro-sphingosine + ?
-
 ?
3.5.1.23 D-erythro-C12-4-nitrobenzo-2-oxa-1,3-diazole-phytoceramide + H2O substrate activity assay Oryza sativa D-erythro-sphingosine + ?
-
 ?
3.5.1.23 palmitoyl sphingosine + H2O substrate activity assay Oryza sativa palmitate + sphingosine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.23 ceramidase
-
 Oryza sativa
3.5.1.23 OsCDase
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.23 37
-
 activity assay Oryza sativa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.23 5.7 6
-
 Oryza sativa