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Literature summary extracted from

  • Neelakandan, A.K.; Song, Z.; Wang, J.; Richards, M.H.; Wu, X.; Valliyodan, B.; Nguyen, H.T.; Nes, W.D.
    Cloning, functional expression and phylogenetic analysis of plant sterol 24C-methyltransferases involved in sitosterol biosynthesis (2009), Phytochemistry, 70, 1982-1998.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.142 expression in Escherichia coli Glycine max
2.1.1.143 expression in Escherichia coli Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.142 24(28)-methylene cycloartanol competitive Glycine max
2.1.1.142 25-azacycloartanol non-competitive Glycine max
2.1.1.142 25-azacyloartenol
-
 Glycine max
2.1.1.142 sitosterol competitive Glycine max
2.1.1.143 24(28)-methylene cycloartanol
-
 Glycine max
2.1.1.143 25-Azacycloartenol
-
 Glycine max
2.1.1.143 sitosterol
-
 Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.142 0.024
-
 Cycloartenol
-
 Glycine max
2.1.1.142 0.028
-
 24(28)methylene lophenol
-
 Glycine max
2.1.1.142 0.03
-
 Cycloartenol
-
 Glycine max
2.1.1.142 0.03
-
 24(28)-methylene lophenol
-
 Glycine max
2.1.1.143 0.024
-
 Cycloartenol pH 7.5, 35°C Glycine max
2.1.1.143 0.028
-
 24(28)-methylene lophenol pH 7.5, 35°C Glycine max
2.1.1.143 0.03
-
 24(28)-methylene lophenol pH 7.5, 35°C Glycine max
2.1.1.143 0.035
-
 Cycloartenol pH 7.5, 35°C Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.142 40000
-
 4 * 40340, calculated, 4 * 40000, SDS-PAGE Glycine max
2.1.1.142 40340
-
 4 * 40340, calculated, 4 * 40000, SDS-PAGE Glycine max
2.1.1.142 40397
-
 x * 40397, calculated Glycine max
2.1.1.142 160000
-
 gel filtration Glycine max
2.1.1.143 40000
-
 4 * 40400, calculated, 4 * 40000, SDS-PAGE Glycine max
2.1.1.143 40300
-
 x * 40300, calculated Glycine max
2.1.1.143 40400
-
 4 * 40400, calculated, 4 * 40000, SDS-PAGE Glycine max
2.1.1.143 160000
-
 gel filtration Glycine max

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.142 Glycine max
-
 isoform SMT2-1
-
2.1.1.142 Glycine max D2D5G4 isoform SMT2-2
-
2.1.1.142 Glycine max Q43445
-
-
2.1.1.143 Glycine max D2D5G3 isoform SMT2-1
-
2.1.1.143 Glycine max D2D5G4 isoform SMT2-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.142 isoform SMT2-2 is purified to homogeneity by column chromatography, gel filtration Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.142 leaf
-
 Glycine max
-
2.1.1.142 leaf young leaf Glycine max
-
2.1.1.143 leaf
-
 Glycine max
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.142 24(28)-methylene lophenol + S-adenosyl-L-methionine
-
 Glycine max ?
-
 ?
2.1.1.142 24(28)-methylene lophenol + S-adenosyl-L-methionine
-
 Glycine max 24(28)Z-ethylidene lophenol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.142 cycloartenol + S-adenosyl-L-methionine
-
 Glycine max 24-methylenecycloartanol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.142 cycloartenol + S-adenosyl-L-methionine 64% of the effectiveness with 24(28)-methylene lophenol Glycine max 24-methylenecycloartanol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.142 cycloartenol + S-adenosyl-L-methionine optimal substrate for SMT1 Glycine max 24(28)-methylene cycloartanol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.142 ergosta-7,24(28)-dienol + S-adenosyl-L-methionine fecosterol and ergosta-7,24(28)-dienol which lack C4-and C14-methyl groups in the nucleus are catalyzed equally effectively by SMT2-2 as the natural substrate 24(28)-methylene lophenol Glycine max ? + S-adenosyl-L-homocysteine
-
 ?
2.1.1.142 fecosterol + S-adenosyl-L-methionine fecosterol and ergosta-7,24(28)-dienol which lack C4-and C14-methyl groups in the nucleus are catalyzed equally effectively by SMT2-2 as the natural substrate 24(28)-methylene lophenol Glycine max ? + S-adenosyl-L-homocysteine
-
 ?
2.1.1.142 lanosterol + S-adenosyl-L-methionine
-
 Glycine max ? + S-adenosyl-L-homocysteine
-
 ?
2.1.1.142 zymosterol + S-adenosyl-L-methionine
-
 Glycine max fecosterol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.143 S-adenosyl-L-methionine + 24(28)-methylene lophenol best substrate Glycine max S-adenosyl-L-homocysteine + 24(28)-ethylidene lophenol
-
 ?
2.1.1.143 S-adenosyl-L-methionine + 4alpha-methyl zymosterol 60% of the effectiveness of 24(28)-methylene lophenol Glycine max S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.143 S-adenosyl-L-methionine + cycloartenol
-
 Glycine max S-adenosyl-L-homocysteine + 24-methylenecycloartanol
-
 ?
2.1.1.143 S-adenosyl-L-methionine + ergosta-7,24(28)-dienol equally effective as 24(28)-methylene lophenol Glycine max S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.143 S-adenosyl-L-methionine + fecosterol equally effective as 24(28)-methylene lophenol Glycine max S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.143 S-adenosyl-L-methionine + zymosterol 70% of the effectiveness of 24(28)-methylene lophenol Glycine max S-adenosyl-L-homocysteine + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.142 ? x * 40397, calculated Glycine max
2.1.1.142 tetramer 4 * 40340, calculated, 4 * 40000, SDS-PAGE Glycine max
2.1.1.143 ? x * 40300, calculated Glycine max
2.1.1.143 tetramer 4 * 40400, calculated, 4 * 40000, SDS-PAGE Glycine max

Synonyms

EC Number Synonyms Comment Organism
2.1.1.142 SMT1
-
 Glycine max
2.1.1.142 sterol 24C-methyltransferases
-
 Glycine max
2.1.1.142 zymosterol-24(25)-methyltransferase
-
 Glycine max
2.1.1.143 sterol 24C-methyltransferase
-
 Glycine max

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.142 0.01
-
 Cycloartenol
-
 Glycine max
2.1.1.142 0.013
-
 24(28)methylene lophenol
-
 Glycine max
2.1.1.143 0.013
-
 24(28)-methylene lophenol pH 7.5, 35°C Glycine max
2.1.1.143 0.022
-
 24(28)-methylene lophenol pH 7.5, 35°C Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.142 7.5
-
-
 Glycine max

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.142 S-adenosyl-L-methionine
-
 Glycine max

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.142 0.00001
-
 25-azacycloartanol substrate cycloartenol Glycine max
2.1.1.142 0.00002
-
 25-azacycloartanol substrate 24(28)-methylene lophenol Glycine max
2.1.1.142 0.00004
-
 25-azacyloartenol
-
 Glycine max
2.1.1.142 0.00009
-
 24(28)-methylene cycloartanol
-
 Glycine max
2.1.1.142 0.0001
-
 sitosterol
-
 Glycine max
2.1.1.142 0.077
-
 24(28)-methylene cycloartanol substrate cycloartenol Glycine max
2.1.1.142 0.1
-
 sitosterol substrate cycloartenol Glycine max
2.1.1.142 0.116
-
 24(28)-methylene cycloartanol substrate 24(28)-methylene lophenol Glycine max
2.1.1.142 0.154
-
 sitosterol substrate 24(28)-methylene lophenol Glycine max
2.1.1.143 0.00001
-
 25-Azacycloartenol substrate cycloartenol, pH 7.5, 35°C Glycine max
2.1.1.143 0.00002
-
 25-Azacycloartenol substrate 24(28)-methylene lophenol, pH 7.5, 35°C Glycine max
2.1.1.143 0.00002
-
 25-Azacycloartenol substrate cycloartenol, pH 7.5, 35°C Glycine max
2.1.1.143 0.077
-
 24(28)-methylene cycloartanol substrate cycloartenol, pH 7.5, 35°C Glycine max
2.1.1.143 0.1
-
 sitosterol substrate cycloartenol, pH 7.5, 35°C Glycine max
2.1.1.143 0.116
-
 24(28)-methylene cycloartanol substrate 24(28)-methylene lophenol, pH 7.5, 35°C Glycine max
2.1.1.143 0.154
-
 sitosterol substrate 24(28)-methylene lophenol, pH 7.5, 35°C Glycine max
2.1.1.143 0.2
-
 sitosterol substrate cycloartenol, pH 7.5, 35°C Glycine max
2.1.1.143 0.25
-
 24(28)-methylene cycloartanol substrate cycloartenol, pH 7.5, 35°C Glycine max
2.1.1.143 0.297
-
 sitosterol substrate 24(28)-methylene lophenol, pH 7.5, 35°C Glycine max

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.142 Glycine max calculated
-
 6.3
2.1.1.142 Glycine max calculated
-
 6.5
2.1.1.143 Glycine max calculated
-
 6.3

General Information

EC Number General Information Comment Organism
2.1.1.143 physiological function SMT isoform expression determines specific C24-methyl to C24-ethyl ratios to flowering whereas with seed development there is a disconnection such that the SMT transcript levels decrease against an increase in sterol content. Generally SMT2-2 is expressed more than SMT2-1 or SMT1 Glycine max