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Literature summary extracted from
- The Arabidopsis At1g45130 and At3g52840 genes encode beta-galactosidases with activity toward cell wall polysaccharides (2008), Phytochemistry, 69, 1661-1670.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.23 | expression in Pichia pastoris | Arabidopsis thaliana |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.23 | cell wall | associated with the cell wall | Arabidopsis thaliana | 5618 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.23 | 75000 | - |
x * 79000, calculated, x * 75000, SDS-PAGE | Arabidopsis thaliana |
| 3.2.1.23 | 75000 | - |
x * 81000, calculated, x * 75000, SDS-PAGE | Arabidopsis thaliana |
| 3.2.1.23 | 79000 | - |
x * 79000, calculated, x * 75000, SDS-PAGE | Arabidopsis thaliana |
| 3.2.1.23 | 81000 | - |
x * 81000, calculated, x * 75000, SDS-PAGE | Arabidopsis thaliana |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.23 | Arabidopsis thaliana | Q9LFA6 | - |
- |
| 3.2.1.23 | Arabidopsis thaliana | Q9MAJ7 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.23 | recombinant protein | Arabidopsis thaliana |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.23 | additional information | low expression in root and silique | Arabidopsis thaliana | - |
| 3.2.1.23 | rosette leaf | - |
Arabidopsis thaliana | - |
| 3.2.1.23 | stem | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.23 | 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Arabidopsis thaliana | 2-nitrophenol + D-galactose | 61% of the activity with 4-nitrophenyl-beta-D-galactopyranoside | ? |  |
| 3.2.1.23 | 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Arabidopsis thaliana | 2-nitrophenol + D-galactose | 72% of the activity with 4-nitrophenyl-beta-D-galactopyranoside | ? | |
| 3.2.1.23 | 4-methylumbelliferyl-beta-D-galactoside + H2O | - |
Arabidopsis thaliana | 4-methylumbelliferone + beta-D-galactose | 18% of the activity with 4-nitrophenyl-beta-D-galactopyranoside | ? | |
| 3.2.1.23 | 4-methylumbelliferyl-beta-D-galactoside + H2O | - |
Arabidopsis thaliana | 4-methylumbelliferone + beta-D-galactose | 20% of the activity with 4-nitrophenyl-beta-D-galactopyranoside | ? | |
| 3.2.1.23 | 4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Arabidopsis thaliana | 4-nitrophenol + D-galactose | - |
? | |
| 3.2.1.23 | 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O | - |
Arabidopsis thaliana | 5-bromo-4-chloroindol + D-galactose | 43% of the activity with 4-nitrophenyl-beta-D-galactopyranoside | ? | |
| 3.2.1.23 | 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O | - |
Arabidopsis thaliana | 5-bromo-4-chloroindol + D-galactose | 56% of the activity with 4-nitrophenyl-beta-D-galactopyranoside | ? | |
| 3.2.1.23 | 6-bromo-2-naphthyl-beta-galactoside + H2O | - |
Arabidopsis thaliana | 6-bromo-2-naphthol + D-galactose | 22% of the activity with 4-nitrophenyl-beta-D-galactopyranoside | ? | |
| 3.2.1.23 | 6-bromo-2-naphthyl-beta-galactoside + H2O | - |
Arabidopsis thaliana | 6-bromo-2-naphthol + D-galactose | 30% of the activity with 4-nitrophenyl-beta-D-galactopyranoside | ? | |
| 3.2.1.23 | additional information | enzyme shows a broad specificity for the aglycone moiety and a strict specificity for the glycone moiety preferring galactose and fucose in the substrate. Enzyme cleaves beta-(1,4) and beta-(1,3) linkages in galacto-oligosaccharides and hydrolyzes the pectic fraction of Arabidopsis cell wall | Arabidopsis thaliana | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.23 | ? | x * 79000, calculated, x * 75000, SDS-PAGE | Arabidopsis thaliana |
| 3.2.1.23 | ? | x * 81000, calculated, x * 75000, SDS-PAGE | Arabidopsis thaliana |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.23 | Gal-2 | - |
Arabidopsis thaliana |
| 3.2.1.23 | Gal-5 | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.23 | 40 | - |
- |
Arabidopsis thaliana |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.23 | 50 | - |
stable up to | Arabidopsis thaliana |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.23 | 4 | 4.5 | - |
Arabidopsis thaliana |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.23 | Arabidopsis thaliana | calculated | - |
8.1 |
| 3.2.1.23 | Arabidopsis thaliana | calculated | - |
8.6 |
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