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Literature summary extracted from

  • Lotfy, S.; Negrel, J.; Javelle, F.
    Formation of omega-feruloyloxypalmitic acid by an enzyme from wound-healing potato tuber disks (1994), Phytochemistry, 35, 1419-1424.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.188 Tris-HCl 60% of maximal activity in Tris-HCl compared with potassium phosphate buffer Solanum tuberosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.188 0.001
-
 16-hydroxypalmitic acid pH 7.0, 30°C Solanum tuberosum
2.3.1.188 0.0017
-
 hexadecan-1,16-diol pH 7.0, 30°C Solanum tuberosum
2.3.1.188 0.004
-
 1-Tetradecanol pH 7.0, 30°C Solanum tuberosum
2.3.1.188 0.005
-
 feruloyl-CoA pH 7.0, 30°C Solanum tuberosum
2.3.1.188 0.005
-
 1-Dodecanol pH 7.0, 30°C Solanum tuberosum
2.3.1.188 0.0071
-
 sinapoyl-CoA pH 7.0, 30°C Solanum tuberosum
2.3.1.188 0.0095
-
 1-pentadecanol pH 7.0, 30°C Solanum tuberosum
2.3.1.188 0.01
-
 1-hexadecanol pH 7.0, 30°C Solanum tuberosum
2.3.1.188 0.0143
-
 p-Coumaroyl-CoA pH 7.0, 30°C Solanum tuberosum
2.3.1.188 0.0168
-
 15-hydroxypentadecanoic acid pH 7.0, 30°C Solanum tuberosum

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.188 Solanum tuberosum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.188
-
 Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.188 tuber wound-healing tuber disc Solanum tuberosum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.188 feruloyl-CoA + 1-dodecanol 49% of the activity with 16-hydroxypalmitic acid Solanum tuberosum CoA + (E)-dodecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
 ?
2.3.1.188 feruloyl-CoA + 1-hexadecanol 52% of the activity with 16-hydroxypalmitic acid Solanum tuberosum CoA + (E)-hexadecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
 ?
2.3.1.188 feruloyl-CoA + 1-pentadecanol 53% of the activity with 16-hydroxypalmitic acid Solanum tuberosum CoA + (E)-pentadecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
 ?
2.3.1.188 feruloyl-CoA + 1-tetradecanol 82% of the activity with 16-hydroxypalmitic acid Solanum tuberosum CoA + (E)-tetradecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
 ?
2.3.1.188 feruloyl-CoA + 15-hydroxypentadecanoic acid 68% of the activity with 16-hydroxypalmitate Solanum tuberosum CoA + (E)-15-(3-(4-hydroxy-3-methoxyphenyl)acryloyloxy)pentadecanoic acid
-
 ?
2.3.1.188 feruloyl-CoA + 16-hydroxypalmitic acid
-
 Solanum tuberosum CoA + (E)-16-(3-(4-hydroxy-3-methoxyphenyl)acryloyloxy)hexadecanoic acid
-
 ?
2.3.1.188 feruloyl-CoA + hexadecane-1,16-diol 52% of the activity with 16-hydroxypalmitic acid Solanum tuberosum CoA + (E)-16-hydroxyhexadecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate no formation of the diferulate ?
2.3.1.188 p-coumaroyl-CoA + 16-hydroxypalmitic acid 51% of the activity with feruloyl-CoA Solanum tuberosum CoA + (E)-16-(3-(4-hydroxyphenyl)acryloyloxy)hexadecanoic acid
-
 ?
2.3.1.188 sinapoyl-CoA + 16-hydroxypalmitic acid 50% of the activity with feruloyl-CoA Solanum tuberosum CoA + (E)-16-(3-(4-hydroxy-3,5-dimethoxyphenyl)acryloyloxy)hexadecanoic acid
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.188 7
-
 potassium phosphate buffer, 60% of maximal activity in Tris-HCl Solanum tuberosum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.188 5.5
-
 half-maximal activity Solanum tuberosum
2.3.1.188 7.5
-
 half-maximal activity Solanum tuberosum
2.3.1.188 10
-
 5% of maximal acitvity Solanum tuberosum