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Literature summary extracted from

  • Bielawski, K.; Bielawska, A.; S?odownik, T.; Bo?kun-Skornicka, U.; Muszy?ska, A.
    Proline-linked nitrosoureas as prolidase-convertible prodrugs in human breast cancer cells (2008), Pharmacol. Rep., 60, 171-182.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.13.9 N-[N'-(2-bromoethyl)-N'-nitrosocarbamoyl]-L-proline significantly increases MCF-7 cells prolidase activity, when used at 0.05-0.25 mM concentrations Homo sapiens
3.4.13.9 N-[N'-(2-chloroethyl)-N'-nitrosocarbamoyl]-L-proline significantly increases MCF-7 cells prolidase activity, when used at 0.05-0.25 mM concentrations Homo sapiens
3.4.13.9 N-[N'-(3-chloropropyl)-N'-nitrosocarbamoyl]-L-proline significantly increases MCF-7 cells prolidase activity, when used at 0.05-0.25 mM concentrations Homo sapiens
3.4.13.9 N-[N'-(4-bromophenyl)-N'-nitrosocarbamoyl]-L-proline significantly increases MCF-7 cells prolidase activity, when used at 0.05-0.25 mM concentrations Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.13.9 carmustine
-
 Sus scrofa
3.4.13.9 additional information carmustine does not inhibit MCF-7 cells prolidase activity Homo sapiens
3.4.13.9 N-[N'-(2-bromoethyl)-N'-nitrosocarbamoyl]-L-proline
-
 Sus scrofa
3.4.13.9 Z-Pro roughly 80% inhibition at 2.53 mM Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.13.9 cytosol
-
 Homo sapiens 5829
-
3.4.13.9 cytosol
-
 Sus scrofa 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.13.9 Mn2+ activation of prolidase requires preincubation with 2 mM Mn2+ Homo sapiens
3.4.13.9 Mn2+ activation of prolidase requires preincubation with 2 mM Mn2+ Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.13.9 56000
-
 SDS-PAGE Homo sapiens
3.4.13.9 56000
-
 SDS-PAGE Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.9 Homo sapiens
-
-
-
3.4.13.9 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.13.9 breast cancer cell
-
 Homo sapiens
-
3.4.13.9 kidney
-
 Sus scrofa
-
3.4.13.9 MCF-7 cell
-
 Homo sapiens
-
3.4.13.9 MDA-MB-231 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.13.9 Gly-L-Pro + H2O
-
 Homo sapiens Gly + L-Pro
-
 ?
3.4.13.9 Gly-L-Pro + H2O
-
 Sus scrofa Gly + L-Pro
-
 ?
3.4.13.9 glycyl-L-hydroxyproline + H2O
-
 Homo sapiens Gly + L-hydroxyproline
-
 ?
3.4.13.9 glycyl-L-hydroxyproline + H2O
-
 Sus scrofa Gly + L-hydroxyproline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.13.9 prolidase
-
 Homo sapiens
3.4.13.9 prolidase
-
 Sus scrofa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.13.9 0.038
-
 in 0.05 M Tris-HCl, pH 7.8, 2 mM MnCl2, at 37°C Sus scrofa carmustine
3.4.13.9 0.082
-
 in 0.05 M Tris-HCl, pH 7.8, 2 mM MnCl2, at 37°C Sus scrofa N-[N'-(2-bromoethyl)-N'-nitrosocarbamoyl]-L-proline