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Literature summary extracted from

  • Larrea, A.A.; Pedroso, I.M.; Malhotra, A.; Myers, R.S.
    Identification of two conserved aspartic acid residues required for DNA digestion by a novel thermophilic Exonuclease VII in Thermotoga maritima (2008), Nucleic Acids Res., 36, 5992-6003.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.11.6 additional information acetate has no effect Escherichia coli
3.1.11.6 phosphate stimulates Escherichia coli
3.1.11.6 sulfate stimulates Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.11.6 wild-type and mutant ExoVII (XseA/B homologs TM1768 and TM1769) coexpressed in Escherichia coli BL21(DE3) cells Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
3.1.11.6 D235A/D240A is completely inactive, even in the presence of magnesium Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.11.6 EDTA completely inhibits Thermotoga maritima
3.1.11.6 additional information is not active in standard Escherichia coli ExoVII buffer. Acetate has no effect Thermotoga maritima
3.1.11.6 phosphate inhibits nuclease activity at 80°C; strongly inhibits, almost complete inhibition by 50 mM Thermotoga maritima
3.1.11.6 sulfate strongly inhibits, almost complete inhibition by 50 mM Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.11.6 KCl ExoVII activity is maximal at low salt concentrations (lower than 250 mM) Thermotoga maritima
3.1.11.6 Mg2+ robust nuclease activity at 80°C is magnesium dependent Thermotoga maritima
3.1.11.6 additional information manganese, nickel, cobalt and zinc have no effect Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.1.11.6 Escherichia coli
-
-
-
3.1.11.6 Thermotoga maritima
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.11.6 wild-type and mutant ExoVII (XseA/B homologs TM1768 and TM1769) purified by gel filtration and on nickel-NTA column Thermotoga maritima
3.1.11.6 wild-type and mutant ExoVII (XseA/B homologs TM1768 and TM1769) purified on nickel-NTA column Thermotoga maritima

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.11.6 221
-
-
 Thermotoga maritima
3.1.11.6 267
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.11.6 additional information active site motif with two conserved aspartate residues in the large (XseA/TM1768) subunit. Residues Asp235 and Asp240 are essential for the nuclease activity Thermotoga maritima ?
-
 ?
3.1.11.6 additional information no significant activity on dsDNA. Active site motif with two conserved aspartate residues in the large (XseA/TM1768) subunit. Residues Asp235 and Asp240 are essential for the nuclease activity Thermotoga maritima ?
-
 ?
3.1.11.6 single-stranded DNA + H2O ExoVII activity is highly processive. It binds ssDNA ends and cleaves its substrate endonucleolytically, releasing oligonucleotide products of various sizes Thermotoga maritima ?
-
 ?
3.1.11.6 single-stranded DNA + H2O releases oligonucleotide products Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.11.6 More stoichiometry of small to large subunits regulates ExoVII activity Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
3.1.11.6 exonuclease VII
-
 Escherichia coli
3.1.11.6 exonuclease VII
-
 Thermotoga maritima
3.1.11.6 XseA
-
 Thermotoga maritima
3.1.11.6 XseB
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.11.6 80
-
 XseA/B homologs TM1768 and TM1769 show robust and maximal nuclease activity Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.11.6 80
-
 XseA/B homologs TM1768 and TM1769 show robust nuclease activity Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.11.6 7.9
-
-
 Thermotoga maritima