EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.21.3.3 | the crystal structures of berberine bridge enzyme in two different crystal forms, monoclinic and tetragonal, and in complex with (S)-reticuline are determined | Eschscholzia californica |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.21.3.3 | E417Q | mutant, based on structural information, Glu417 essential amino acid for substrate oxidation, bicovalent flavin linkage is not affected by the mutation | Eschscholzia californica |
1.21.3.3 | H459A | mutant, based on structural information, His459 do not directly interact with the substrate, bicovalent flavin linkage is not affected by the mutation | Eschscholzia californica |
1.21.3.3 | Y106F | mutant, based on structural information, Tyr106 do not directly interact with the substrate, bicovalent flavin linkage is not affected by the mutation | Eschscholzia californica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.21.3.3 | (S)-reticuline + O2 | Eschscholzia californica | - |
(S)-scoulerine + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.21.3.3 | Eschscholzia californica | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.21.3.3 | (S)-reticuline + O2 | - |
Eschscholzia californica | (S)-scoulerine + H2O2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.21.3.3 | BBE | - |
Eschscholzia californica |
1.21.3.3 | berberine bridge enzyme | - |
Eschscholzia californica |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.21.3.3 | 0.054 | - |
(S)-reticuline | mutant E417Q | Eschscholzia californica | |
1.21.3.3 | 0.7 | - |
(S)-reticuline | mutant Y106F | Eschscholzia californica | |
1.21.3.3 | 3.1 | - |
(S)-reticuline | mutant H459A | Eschscholzia californica | |
1.21.3.3 | 8 | - |
(S)-reticuline | wild-type enzyme | Eschscholzia californica |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.21.3.3 | FAD | - |
Eschscholzia californica |