EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.78 | Salmonella typhimurium SL1344 wild-type and deltaSigD mutant lacking SigD. Transfection of COS-7 and HeLa cells with SigD in mammalian expression vectors. Visualization of phosphatidylinositol 4,5-bisphosphate during Salmonella infection. Fusion protein of PH domain of phospholipase Cdelta and GFP (PLCdelta-PH-GFP) during invasion of HeLA cells by Salmonella (confocal fluorescence microscopy): phosphatidylinositol 4,5-bisphosphate is abundant near the tip but disappears from the basal regions. Role of SigD in phosphatidylinositol 4,5-bisphosphate elimination: focal disappearance of phosphatidylinositol 4,5-bisphosphate observed during invasion (wild-type) is diminished and delayed in the deltaSigD mutant (confocal fluorescence microscopy). SigD alters memebrane elasticity (atomic force microscopy, SigD transfected HeLa cells) and induces vacuole formation. SigD promotes membrane fission during invasion (SigD transfected HeLa cells) | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.3.78 | C462S | does not restore the ability of Salmonella to eliminate phosphatidylinositol 4,5-bisphosphate in SigD-deficient bacteria | Salmonella enterica subsp. enterica serovar Typhimurium |
3.1.3.78 | K527A | restores the ability of Salmonella to eliminate phosphatidylinositol 4,5-bisphosphate in SigD-deficient bacteria | Salmonella enterica subsp. enterica serovar Typhimurium |
3.1.3.78 | K530A | restores the ability of Salmonella to eliminate phosphatidylinositol 4,5-bisphosphate in SigD-deficient bacteria | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.78 | phosphatidylinositol 3,4,5-trisphosphate + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
phosphatidylinositol 3,5-bisphosphate + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.78 | Salmonella enterica subsp. enterica serovar Typhimurium | O30916 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.78 | phosphatidylinositol 3,4,5-trisphosphate + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | phosphatidylinositol 3,5-bisphosphate + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.78 | SigD | - |
Salmonella enterica subsp. enterica serovar Typhimurium |