EC Number | Cloned (Comment) | Organism |
---|---|---|
7.6.2.2 | expression of wild-type an dmutant enzymes in HEK-293 cells | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
7.6.2.2 | D1179A | site-directed mutagenesis, the mutant shows similar or higher expression level, but about 35% reduced drug transport activity and altered substrate specificity compared to the wild-type enzyme | Homo sapiens |
7.6.2.2 | D1183E | site-directed mutagenesis, the mutant shows transport activities similar to the wild-type enzyme, but reduced expression level | Homo sapiens |
7.6.2.2 | E1144A | site-directed mutagenesis, the mutant shows similar or higher expression level, but about 50% reduced drug transport activity compared to the wild-type enzyme | Homo sapiens |
7.6.2.2 | E1184A | site-directed mutagenesis, the mutant shows similar or higher expression level, but reduced activity and altered substrate specificity compared to the wild-type enzyme | Homo sapiens |
7.6.2.2 | K1181A | site-directed mutagenesis, the mutant shows similar or higher expression level compared to the wild-type enzyme | Homo sapiens |
7.6.2.2 | R1166K | site-directed mutagenesis, the mutant shows transport activities similar to the wild-type enzyme, but reduced expression level | Homo sapiens |
7.6.2.2 | R1173A | site-directed mutagenesis, the mutant shows similar or higher expression level, but increased activity and altered substrate specificity compared to the wild-type enzyme | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
7.6.2.2 | membrane | - |
Homo sapiens | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
7.6.2.2 | Mg2+ | - |
Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.6.2.2 | ATP + H2O + xenobiotic/in | Homo sapiens | - |
ADP + phosphate + xenobiotic/out | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
7.6.2.2 | Homo sapiens | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
7.6.2.2 | additional information | - |
relative activities and substrate specificity of wild-type an dmutant enzymes | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.6.2.2 | 8-azido-ATP + H2O + xenobiotic/in | - |
Homo sapiens | 8-azido-ADP + phosphate + xenobiotic/out | - |
? | |
7.6.2.2 | ATP + H2O + 17beta-estradiol 17-(beta-D-glucuronide)/in | - |
Homo sapiens | ADP + phosphate + 17beta-estradiol 17-(beta-D-glucuronide)/out | - |
? | |
7.6.2.2 | ATP + H2O + estrone-3-sulfate/in | - |
Homo sapiens | ADP + phosphate + estrone-3-sulfate/out | - |
? | |
7.6.2.2 | ATP + H2O + glutathione/in | - |
Homo sapiens | ADP + phosphate + glutathione/out | - |
? | |
7.6.2.2 | ATP + H2O + leukotriene C4/in | - |
Homo sapiens | ADP + phosphate + leukotriene C4/out | - |
? | |
7.6.2.2 | ATP + H2O + methotrexate/in | - |
Homo sapiens | ADP + phosphate + methotrexate/out | - |
? | |
7.6.2.2 | ATP + H2O + xenobiotic/in | - |
Homo sapiens | ADP + phosphate + xenobiotic/out | - |
? | |
7.6.2.2 | ATP + H2O + xenobiotic/in | ATP-dependent MRP1-mediated uptake of labeled substrates in membrane vesicles | Homo sapiens | ADP + phosphate + xenobiotic/out | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
7.6.2.2 | ABCC1 | - |
Homo sapiens |
7.6.2.2 | MRP1 | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
7.6.2.2 | 37 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
7.6.2.2 | 7.5 | - |
assay at | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
7.6.2.2 | ATP | - |
Homo sapiens |