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Literature summary extracted from

  • Kawai, H.; Tanji, T.; Shiraishi, H.; Yamada, M.; Iijima, R.; Inoue, T.; Kezuka, Y.; Ohashi, K.; Yoshida, Y.; Tohyama, K.; Gengyo-Ando, K.; Mitani, S.; Arai, H.; Ohashi-Kobayashi, A.; Maeda, M.
    Normal formation of a subset of intestinal granules in Caenorhabditis elegans requires ABC transporters HAF-4 and HAF-9, which are highly homologous to human lysosomal peptide transporter TAPL (TAP-like) (2009), Mol. Biol. Cell, 20, 2979-2990.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.4.2.5 genes haf-4 and haf-5, phylogenetic tree, overexpression of wild-type and mutant GFP-tagged enzymes Caenorhabditis elegans
7.4.2.5 phylogenetic tree Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
7.4.2.5 K539M introduction of a Walker A mutation in the haf-4 construct. The mutation in ATP-binding site of HAF-4 inhibits the maturation of granules as dominant-negative effects. The inactive form of HAF-4 does not rescue the phenotype Caenorhabditis elegans
7.4.2.5 additional information the loss-of-function mutants for haf-4 and haf-9, constructed by RNAi mechanism, exhibit granular defects in late larval and young adult intestinal cells, associated with decreased brood size, prolonged defecation cycle, and slow growth. The intestinal granular phenotype is rescued by the overexpression of the GFP-tagged wild-type protein, but not by the ATP-unbound form of HAF-4, overview Caenorhabditis elegans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.4.2.5 lysosomal membrane
-
 Homo sapiens 5765
-
7.4.2.5 membrane
-
 Caenorhabditis elegans 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.4.2.5 ATP + H2O + peptide/in Caenorhabditis elegans
-
 ADP + phosphate + peptide/out
-
 ?
7.4.2.5 ATP + H2O + peptide/in Homo sapiens TAPL is a half-type ABC transporter that localizes in lysosome and putatively conveys peptides from cytosol to lysosome ADP + phosphate + peptide/out
-
 ?
7.4.2.5 additional information Caenorhabditis elegans Caenorhabditis elegans requires ABC transporters HAF-4 and HAF-9, which are highly homologous to human lysosomal peptide transporter TAPL ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.4.2.5 Caenorhabditis elegans
-
 genes haf-4 and haf-9
-
7.4.2.5 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.4.2.5 mutant and wild-type enzymes partially by membrane fractionation Caenorhabditis elegans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.4.2.5 intestine tissue-specific expression of genes haf-4 and haf-9: both recombinant GFP-tagged HAF-4 and HAF-9 are mainly localized on the membrane of non-acidic but LAMP homologue-positive intestinal granules from larval to adult stage Caenorhabditis elegans
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.4.2.5 ATP + H2O + peptide/in
-
 Homo sapiens ADP + phosphate + peptide/out
-
 ?
7.4.2.5 ATP + H2O + peptide/in
-
 Caenorhabditis elegans ADP + phosphate + peptide/out
-
 ?
7.4.2.5 ATP + H2O + peptide/in TAPL is a half-type ABC transporter that localizes in lysosome and putatively conveys peptides from cytosol to lysosome Homo sapiens ADP + phosphate + peptide/out
-
 ?
7.4.2.5 additional information Caenorhabditis elegans requires ABC transporters HAF-4 and HAF-9, which are highly homologous to human lysosomal peptide transporter TAPL Caenorhabditis elegans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.4.2.5 ABCB9
-
 Homo sapiens
7.4.2.5 HAF-4
-
 Caenorhabditis elegans
7.4.2.5 HAF-9
-
 Caenorhabditis elegans
7.4.2.5 lysosomal peptide transporter TAPL
-
 Homo sapiens
7.4.2.5 TAP-like
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
7.4.2.5 ATP
-
 Homo sapiens
7.4.2.5 ATP
-
 Caenorhabditis elegans