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Literature summary extracted from

  • Loutet, S.A.; Bartholdson, S.J.; Govan, J.R.; Campopiano, D.J.; Valvano, M.A.
    Contributions of two UDP-glucose dehydrogenases to viability and polymyxin B resistance of Burkholderia cenocepacia (2009), Microbiology, 155, 2029-2039.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.22 gene Ugd(BCAL2946) cloned into the pGEM-T Easy vector, sequenced, and ligated into pET29a vector to transform Escherichia coli Top10 cells. Ugd(BCAL2946) expressed in Escherichia coli HMS 174(DE3) Burkholderia cenocepacia J2315
1.1.1.22 gene Ugd(BCAM0855) cloned into the pGEM-T Easy vector, sequenced, and ligated into pET29a vector to transform Escherichia coli Top10 cells. Ugd(BCAM0855) expressed in Escherichia coli HMS 174(DE3) Burkholderia cenocepacia J2315
1.1.1.22 gene Ugd(BCAM2034) cloned into the pGEM-T Easy vector, sequenced, and ligated into pET29a vector to transform Escherichia coli Top10 cells. Ugd(BCAM2034) expressed in Escherichia coli BL21 (DE3) Burkholderia cenocepacia J2315

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.22 additional information inactivation of gene Ugd(BCAL2946) results in increased sensitivity to polymyxin B and this sensitivity can be overcome when either genes Ugd(BCAL2946) or Ugd(BCAM0855) but not gene ugd(BCAM2034) is expressed from plasmids. Growth of a conditional Ugd(BCAL2946) mutant, created in the DELTAUgd(BCAM0855) background, is significantly impaired under non-permissive conditions. Growth can be rescued by either Ugd(BCAL2946) or Ugd(BCAM0855) expressed in trans, but not by Ugd(BCAM2034) Burkholderia cenocepacia J2315

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.22 0.02
-
 UDP-glucose
-
 Burkholderia cenocepacia J2315
1.1.1.22 0.17
-
 NAD+
-
 Burkholderia cenocepacia J2315
1.1.1.22 0.21
-
 NAD+
-
 Burkholderia cenocepacia J2315

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.22 50900
-
 Ugd-FLAG-BCAM2034 Burkholderia cenocepacia J2315
1.1.1.22 51700
-
 Ugd-FLAG-BCAL2946 Burkholderia cenocepacia J2315
1.1.1.22 52100
-
 Ugd-FLAG-BCAM0855 Burkholderia cenocepacia J2315

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.22 Burkholderia cenocepacia J2315 B4EB36
-
-
1.1.1.22 Burkholderia cenocepacia J2315 B4EMQ8
-
-
1.1.1.22 Burkholderia cenocepacia J2315 B4EN77
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.22 by nickel affinity and gel filtration Burkholderia cenocepacia J2315

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.22 additional information expression of Ugd(BCAL2946) is 5.4- and 135fold greater than that of Ugd(BCAM0855) and Ugd(BCAM2034), respectively. Combined activity of Ugd(BCAL2946) and Ugd(BCAM0855) is essential for the survival of Burkholderia cenocepacia but only the most highly expressed ugd gene, Ugd(BCAL2946), is required for polymyxin B resistance. UDP-galactose, UDP-acetylglucosamine and GDP-mannose are not substrates Burkholderia cenocepacia J2315 ?
-
 ?
1.1.1.22 additional information expression of Ugd(BCAL2946) is 5.4- and 135fold greater than that of Ugd(BCAM0855) and Ugd(BCAM2034), respectively. Combined activity of Ugd(BCAL2946) and Ugd(BCAM0855) is essential for the survival of Burkholderia cenocepacia. UDP-galactose, UDP-acetylglucosamine and GDP-mannose are not substrates Burkholderia cenocepacia J2315 ?
-
 ?
1.1.1.22 additional information purified Ugd(BCAM2034) shows no in vitro Ugd activity. Expression of Ugd(BCAL2946) is 5.4- and 135fold greater than that of Ugd(BCAM0855) and Ugd(BCAM2034), respectively. UDP-galactose, UDP-acetylglucosamine and GDP-mannose are not substrates Burkholderia cenocepacia J2315 ?
-
 ?
1.1.1.22 UDP-glucose + 2 NAD+ + H2O recombinant forms Ugd(BCAL2946) and Ugd(BCAM0855) have similar in vitro Ugd activity Burkholderia cenocepacia J2315 UDP-glucuronate + 2 NADH + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.22 homodimer recombinant form of Ugd(BCAL2946) Burkholderia cenocepacia J2315
1.1.1.22 homodimer recombinant form of Ugd(BCAM0855) Burkholderia cenocepacia J2315

Synonyms

EC Number Synonyms Comment Organism
1.1.1.22 UDP-glucose dehydrogenase
-
 Burkholderia cenocepacia J2315
1.1.1.22 Ugd
-
 Burkholderia cenocepacia J2315

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.22 0.127
-
 UDP-glucose
-
 Burkholderia cenocepacia J2315
1.1.1.22 0.133
-
 UDP-glucose
-
 Burkholderia cenocepacia J2315
1.1.1.22 0.154
-
 NAD+
-
 Burkholderia cenocepacia J2315
1.1.1.22 0.156
-
 NAD+
-
 Burkholderia cenocepacia J2315