Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Vamsee-Krishna, C.; Phale, P.S.
    Carbon source-dependent modulation of NADP-glutamate dehydrogenases in isophthalate-degrading Pseudomonas aeruginosa strain PP4, Pseudomonas strain PPD and Acinetobacter lwoffii strain ISP4 (2008), Microbiology, 154, 3329-3337.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.4 isophthalate
-
 Acinetobacter lwoffii
1.4.1.4 isophthalate
-
 Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.4 Acinetobacter lwoffii
-
 strain ISP4, when grown on isophthalate, Acinetobacter lwoffii strain ISP4, Pseudomonas aeruginosa strain PP4 and Pseudomonas strain PPD show activity of NADP-dependent GDH, while cells grown on glucose, 2fold yeast extract-tryptone broth (2YT) or glutamate show activities of both NAD-dependent GDH and NADP-GDH
-
1.4.1.4 Pseudomonas aeruginosa
-
 strain PP4, when grown on isophthalate, Acinetobacter lwoffii strain ISP4, Pseudomonas aeruginosa strain PP4 and Pseudomonas strain PPD show activity of NADP-dependent GDH, while cells grown on glucose, 2fold yeast extract-tryptone broth (2YT) or glutamate show activities of both NAD-dependent GDH and NADP-GDH
-
1.4.1.4 Pseudomonas aeruginosa
-
 strain PPD, when grown on isophthalate, Acinetobacter lwoffii strain ISP4, Pseudomonas aeruginosa strain PP4 and Pseudomonas strain PPD show activity of NADP-dependent GDH, while cells grown on glucose, 2fold yeast extract-tryptone broth (2YT) or glutamate show activities of both NAD-dependent GDH and NADP-GDH
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.4 additional information
-
 late-exponential-phase cells are used for this study since they show a higher activity of GDH compared with early exponential-phase cells Pseudomonas aeruginosa
1.4.1.4 additional information
-
 late-exponential-phase cells are used for this study since they show a higher activity of GDH compared with early exponential-phase cells Acinetobacter lwoffii

Synonyms

EC Number Synonyms Comment Organism
1.4.1.4 GdhA activity staining, inhibition and thermal stability studies indicate the carbon source-dependent presence of three (GDHA, GDHB and GDHC) forms of NADP-GDH in Pseudomonas aeruginosa strain PPD Pseudomonas aeruginosa
1.4.1.4 GDHB activity staining, inhibition and thermal stability studies indicate the carbon source-dependent presence of three (GDHA, GDHB and GDHC) forms of NADP-GDH in Pseudomonas aeruginosa strain PPD Pseudomonas aeruginosa
1.4.1.4 GDHC activity staining, inhibition and thermal stability studies indicate the carbon source-dependent presence of three (GDHA, GDHB and GDHC) forms of NADP-GDH in Pseudomonas aeruginosa strain PPD Pseudomonas aeruginosa
1.4.1.4 GDHI activity staining, inhibition and thermal stability studies indicate the carbon source-dependent presence of two (GDHI and GDHII) forms of NADP-GDH in Pseudomonas aeruginosa strain PP4 Pseudomonas aeruginosa
1.4.1.4 GDHII activity staining, inhibition and thermal stability studies indicate the carbon source-dependent presence of two (GDHI and GDHII) forms of NADP-GDH in Pseudomonas aeruginosa strain PP4 Pseudomonas aeruginosa
1.4.1.4 GDHP activity staining, inhibition and thermal stability studies indicate the carbon source-dependent presence of one GDHP form of NADP-GDH in Acinetobacter lwoffii strain ISP4 Acinetobacter lwoffii
1.4.1.4 NADP-glutamate dehydrogenase
-
 Pseudomonas aeruginosa
1.4.1.4 NADP-glutamate dehydrogenase
-
 Acinetobacter lwoffii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.4 additional information
-
 time-dependent changes in the activity of NADP-GDH at 60°C are analysed: GDHII loses activity completely within 10 min Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.4 8.5
-
 assay at Pseudomonas aeruginosa
1.4.1.4 8.5
-
 assay at Acinetobacter lwoffii

General Information

EC Number General Information Comment Organism
1.4.1.4 physiological function carbon source-dependent modulation of different forms of NADP-GDH in bacterial strains Acinetobacter lwoffii strain ISP4, Pseudomonas aeruginosa strain PP4 and Pseudomonas strain PPD Pseudomonas aeruginosa
1.4.1.4 physiological function carbon source-dependent modulation of different forms of NADP-GDH in bacterial strains Acinetobacter lwoffii strain ISP4, Pseudomonas aeruginosa strain PP4 and Pseudomonas strain PPD. Time-dependent changes in the activity of NADP-GDH at 60°C are analysed: GDHI from isophthalate- and mHB-grown cells retain 70% and 90% of its activity, respectively Pseudomonas aeruginosa
1.4.1.4 physiological function carbon source-dependent modulation of different forms of NADP-GDH in bacterial strains Acinetobacter lwoffii strain ISP4, Pseudomonas aeruginosa strain PP4 and Pseudomonas strain PPD. Time-dependent changes in the activity of NADP-GDH at 60°C are analysed: In Pseudomonas aeruginosa strain PPD, isophthalate-, glucose-, 2YT- or mHB-grown cells retain 100% of the activity of NADP-GDH, while PPD cells grown on pHB and benzoate show 25% and 40% loss of activity Pseudomonas aeruginosa
1.4.1.4 physiological function carbon source-dependent modulation of different forms of NADP-GDH in bacterial strains Acinetobacter lwoffii strain ISP4, Pseudomonas aeruginosa strain PP4 and Pseudomonas strain PPD. Time-dependent changes in the activity of NADP-GDH at 60°C are analysed: isophthalate-, glucose-, 2YT- or mHB-grown cells retain 100% of the activity of NADP-GDH Acinetobacter lwoffii