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Literature summary extracted from

  • Chen, H.; Xu, H.; Kweon, O.; Chen, S.; Cerniglia, C.
    Functional role of Trp-105 of Enterococcus faecalis azoreductase (AzoA) as resolved by structural and mutational analysis (2008), Microbiology, 154, 2659-2667.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.6 into the vector pET-11a for expression in Escherichia coli BL21-Gold DE3 pLysS cells Enterococcus faecalis

Protein Variants

EC Number Protein Variants Comment Organism
1.7.1.6 W105A mutant, complete loss of both affinity for FMN and enzyme activity Enterococcus faecalis
1.7.1.6 W105F mutant, lower Vmax value, decrease 30.6fold Enterococcus faecalis
1.7.1.6 W105G mutant, complete loss of both affinity for FMN and enzyme activity Enterococcus faecalis
1.7.1.6 W105H mutant, lower Vmax value, decrease 8.2fold Enterococcus faecalis
1.7.1.6 W105Q mutant, lower Vmax value, decrease 68.2fold Enterococcus faecalis
1.7.1.6 W105Y mutant, substitution does not significantly decrease the Vmax of the enzyme, 22% reduction Enterococcus faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.6 0.0247
-
 Methyl red mutant W105F Enterococcus faecalis
1.7.1.6 0.0251
-
 Methyl red mutant W105H Enterococcus faecalis
1.7.1.6 0.0254
-
 Methyl red wild-type enzyme Enterococcus faecalis
1.7.1.6 0.0276
-
 Methyl red mutant W105Y Enterococcus faecalis
1.7.1.6 0.0282
-
 Methyl red mutant W105Q Enterococcus faecalis
1.7.1.6 0.158
-
 NADH wild-type enzyme Enterococcus faecalis
1.7.1.6 0.166
-
 NADH mutant W105Y Enterococcus faecalis
1.7.1.6 0.169
-
 NADH mutant W105Q Enterococcus faecalis
1.7.1.6 0.171
-
 NADH mutant W105H Enterococcus faecalis
1.7.1.6 0.175
-
 NADH mutant W105F Enterococcus faecalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.6 23000
-
 monomer, determined by SDS-PAGE Enterococcus faecalis

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.6 Enterococcus faecalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.6 by anion-exchange chromatography and by gel filtration Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.6 methyl red + NADH + H+
-
 Enterococcus faecalis ? + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.6 homodimer 2 * 23000 Enterococcus faecalis

Synonyms

EC Number Synonyms Comment Organism
1.7.1.6 AzoA
-
 Enterococcus faecalis
1.7.1.6 azoreductase
-
 Enterococcus faecalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.1.6 23
-
 activity assay at room temperature Enterococcus faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.6 7.1
-
 activity assay Enterococcus faecalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.6 FMN
-
 Enterococcus faecalis
1.7.1.6 NADH
-
 Enterococcus faecalis