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Literature summary extracted from

  • Liang, L.; He, X.; Liu, G.; Tan, H.
    The role of a purine-specific nucleoside hydrolase in spore germination of Bacillus thuringiensis (2008), Microbiology, 154, 1333-1340.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.1 expressed in Escherichia coli BL21(DE3) cells Bacillus thuringiensis
3.2.2.2
-
 Bacillus thuringiensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.1 0.399
-
 Inosine in 50 mM HEPES (pH 7.3), at 25°C Bacillus thuringiensis
3.2.2.2 0.399
-
 Inosine
-
 Bacillus thuringiensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.2.1 exosporium
-
 Bacillus thuringiensis 43592
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.1 36200
-
 calculated from amino acid sequence Bacillus thuringiensis

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.1 Bacillus thuringiensis A7UHH1 subspecies Bacillus thuringiensis kurstaki
-
3.2.2.2 Bacillus thuringiensis A7UHH1 subsp. Kurstaki
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.1 Ni-NTA column chromatography Bacillus thuringiensis
3.2.2.2
-
 Bacillus thuringiensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.1 spore
-
 Bacillus thuringiensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.1 1.32
-
 in 50 mM HEPES, pH 7.3, with guanosine as substrate Bacillus thuringiensis
3.2.2.1 11.76
-
 in 50 mM HEPES, pH 7.3, with adenosine as substrate Bacillus thuringiensis
3.2.2.1 57.2
-
 in 50 mM HEPES, pH 7.3, with inosine as substrate Bacillus thuringiensis
3.2.2.2 1.32
-
 guanosine Bacillus thuringiensis
3.2.2.2 11.76
-
 adenosine Bacillus thuringiensis
3.2.2.2 57.2
-
 inosine Bacillus thuringiensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.1 adenosine + H2O
-
 Bacillus thuringiensis D-ribose + adenine
-
 ?
3.2.2.1 guanosine + H2O
-
 Bacillus thuringiensis D-ribose + guanine
-
 ?
3.2.2.1 inosine + H2O
-
 Bacillus thuringiensis D-ribose + hypoxanthine
-
 ?
3.2.2.1 additional information hydrolase activity could not be detected when uridine or cytidine is used as a substrate Bacillus thuringiensis ?
-
 ?
3.2.2.1 additional information IunH can hydrolyse inosine, adenosine and guanosine with the activity order: inosine Bacillus thuringiensis ?
-
 ?
3.2.2.2 adenosine + H2O
-
 Bacillus thuringiensis adenine + D-ribose
-
 ?
3.2.2.2 guanosine + H2O
-
 Bacillus thuringiensis guanine + D-ribose
-
 ?
3.2.2.2 inosine + H2O
-
 Bacillus thuringiensis hypoxanthine + D-ribose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.1 inosine/adenosine/guanosinepreferring NH
-
 Bacillus thuringiensis
3.2.2.1 IunH IunH is important in moderating inosine- or adenosine-induced germination of Bacillus thuringiensis spores Bacillus thuringiensis
3.2.2.1 purine-specific NH
-
 Bacillus thuringiensis
3.2.2.1 purine-specific nucleoside hydrolase
-
 Bacillus thuringiensis
3.2.2.2 purine-specific nucleoside hydrolase
-
 Bacillus thuringiensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.1 80
-
-
 Bacillus thuringiensis
3.2.2.2 80
-
-
 Bacillus thuringiensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.2.1 40 100 the activity is stable over a wide temperature range, the highest activity being at 80°C with high activity being maintained up to 100°C Bacillus thuringiensis
3.2.2.2 70 100 high activity Bacillus thuringiensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.2.1 48.9
-
 Inosine in 50 mM HEPES (pH 7.3), at 25°C Bacillus thuringiensis
3.2.2.2 48.9
-
 Inosine
-
 Bacillus thuringiensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.1 6
-
-
 Bacillus thuringiensis
3.2.2.2 6
-
-
 Bacillus thuringiensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.2.1 5 10 inosine hydrolase activity of the purified recombinant IunH is detected over a wide range of pH values, the highest activity being obtained at pH 6 Bacillus thuringiensis