Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yongkiettrakul, S.; Boonyapakron, K.; Jongkaewwattana, A.; Wanitchang, A.; Leartsakulpanich, U.; Chitnumsub, P.; Eurwilaichitr, L.; Yuthavong, Y.
    Avian influenza A/H5N1 neuraminidase expressed in yeast with a functional head domain (2009), J. Virol. Methods, 156, 44-51.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.18 fusion of gene encoding the neuraminidase N1 head domain residues 63-449 with the Saccharomyces cerevisiae alpha-factor secretion signal and expression in Pichia pastoris unidentified influenza virus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.18 additional information fusion of gene encoding the neuraminidase N1 head domain residues 63-449 with the Saccharomyces cerevisiae alpha-factor secretion signal and expression in Pichia pastoris. Recombinant product is a soluble protein with similar kinetic characteristics as wild-type unidentified influenza virus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.18 oseltamivir carboxylate
-
 unidentified influenza virus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.18 0.018
-
 2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid wild-type, pH 6.5, 37°C unidentified influenza virus
3.2.1.18 0.018
-
 2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid recombinant head domain, pH 6.5, 37°C unidentified influenza virus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.18 unidentified influenza virus
-
 A/Viet Nam/DT-036/2005 type H5N1
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.18 glycoprotein treatment of recombinant N1 head domain with glycopeptidase F generates a 45000 Da product unidentified influenza virus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.18 314
-
 recombinant head domain, pH 6.5, 37°C unidentified influenza virus
3.2.1.18 378
-
 wild-type, pH 6.5, 37°C unidentified influenza virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.18 2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
-
 unidentified influenza virus 4-methylumbelliferone + N-acetylneuraminic acid
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.18 0.0000013
-
 oseltamivir carboxylate wild-type, pH 6.5, 37°C unidentified influenza virus
3.2.1.18 0.0000024
-
 oseltamivir carboxylate recombinant head domain, pH 6.5, 37°C unidentified influenza virus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.18 0.0000121
-
 wild-type, pH 6.5, 37°C unidentified influenza virus oseltamivir carboxylate
3.2.1.18 0.0000182
-
 recombinant head domain, pH 6.5, 37°C unidentified influenza virus oseltamivir carboxylate