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Literature summary extracted from

  • Lundqvist, J.; Elmlund, D.; Heldt, D.; Deery, E.; Soederberg, C.A.; Hansson, M.; Warren, M.; Al-Karadaghi, S.
    The AAA(+) motor complex of subunits CobS and CobT of cobaltochelatase visualized by single particle electron microscopy (2009), J. Struct. Biol., 167, 227-234.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.99.1.3 genes cobN, cobS and cobT, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3) Brucella melitensis
6.6.1.2 subunits separately overexpressed in Escherichia coli BL21star (DE3)pLysS Brucella melitensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.99.1.3 37000
-
 x * 137000, subunit CobN, + x * 37000, subunit CobS, + x * 71000, subunit CobT Brucella melitensis
4.99.1.3 71000
-
 x * 137000, subunit CobN, + x * 37000, subunit CobS, + x * 71000, subunit CobT Brucella melitensis
4.99.1.3 137000
-
 x * 137000, subunit CobN, + x * 37000, subunit CobS, + x * 71000, subunit CobT Brucella melitensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.99.1.3 sirohydrochlorin + Co2+ Brucella melitensis involved in the formation of a octahedrally co-ordinated cobalt ion. In the oxygen-dependent cobalamin, i.e. vitamin B12, biosynthetic pathway, cobalt is inserted into a ring-contracted tetrapyrrole called hydrogenobyrinic acid a,c-diamide, HBAD, by a cobaltochelatase that is constituted by three subunits, CobN, CobS and CobT cobalt-sirohydrochlorin + 2 H+
-
 ?
6.6.1.2 ATP + hydrogenobyrinic acid a,c-diamide + Co2+ Brucella melitensis
-
 ADP + phosphate + cob(II)yrinic acid a,c-diamide + H+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
4.99.1.3 Brucella melitensis
-
 genes cobN, cobS and cobT encoding the tree subunits
-
6.6.1.2 Brucella melitensis
-
 cobaltochelatase consists of three subunits, CobN, CobS and CobT, while subunits CobS and CobT form a chaperone-like complex, characteristic for the AAA+ class of proteins
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.99.1.3 sirohydrochlorin + Co2+ involved in the formation of a octahedrally co-ordinated cobalt ion. In the oxygen-dependent cobalamin, i.e. vitamin B12, biosynthetic pathway, cobalt is inserted into a ring-contracted tetrapyrrole called hydrogenobyrinic acid a,c-diamide, HBAD, by a cobaltochelatase that is constituted by three subunits, CobN, CobS and CobT Brucella melitensis cobalt-sirohydrochlorin + 2 H+
-
 ?
6.6.1.2 ATP + hydrogenobyrinic acid a,c-diamide + Co2+
-
 Brucella melitensis ADP + phosphate + cob(II)yrinic acid a,c-diamide + H+
-
 ir

Subunits

EC Number Subunits Comment Organism
4.99.1.3 More the molecules are arranged in a two-tiered ring structure with the six subunits in each ring organized as a trimer of dimers, subunits CobS and CobT form a chaperone-like complex. Homology modelling of subunit CobS, overview Brucella melitensis
4.99.1.3 oligomer x * 137000, subunit CobN, + x * 37000, subunit CobS, + x * 71000, subunit CobT Brucella melitensis

Synonyms

EC Number Synonyms Comment Organism
4.99.1.3 cobaltochelatase
-
 Brucella melitensis
4.99.1.3 More the enzyme belongs to the AAA+ superfamily of proteins Brucella melitensis
6.6.1.2 cobaltochelatase
-
 Brucella melitensis