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Literature summary extracted from

  • Cheng, Q.; Sandalova, T.; Lindqvist, Y.; Arner, E.S.
    Crystal structure and catalysis of the selenoprotein thioredoxin reductase 1 (2009), J. Biol. Chem., 284, 3998-4008.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.9 expressed in Escherichia coli BL21(DE3) cells Rattus norvegicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.1.9 in the presence of 10 mM NADP+, hanging drop vapor diffusion method, using 15% polyethylene glycol 3350, 12% ethylene glycol, and 0.1 M HEPES, pH 7.5 Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
1.8.1.9 Y116F the mutant protein is not soluble Rattus norvegicus
1.8.1.9 Y116I the mutation decreases sensitivity to inhibition by cisplatin and lowers catalytic efficiency in reduction of thioredoxin compared to the wild type enzyme Rattus norvegicus
1.8.1.9 Y116I the mutation decreases sensitivity to inhibition by cisplatin, lowers catalytic efficiency in reduction of thioredoxin, and increases turnover using 5-hydroxy-1,4-naphthoquinone (juglone) as substrate compared to the wild type enzyme Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.9 cisplatin complete inhibition after 1 h at 0.5 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.9 0.00099
-
 thioredoxin disulfide mutant enzyme Y116I, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 0.00106
-
 thioredoxin disulfide mutant enzyme Y116T, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 0.00143
-
 thioredoxin disulfide wild type enzyme, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 0.00227
-
 5-hydroxy-1,4-naphthoquinone wild type enzyme, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 0.00698
-
 5-hydroxy-1,4-naphthoquinone mutant enzyme Y116T, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 0.02374
-
 5-hydroxy-1,4-naphthoquinone mutant enzyme Y116I, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 0.0906
-
 5,5'-dithiobis(2-nitrobenzoic acid) mutant enzyme Y116T, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 0.094
-
 5,5'-dithiobis(2-nitrobenzoic acid) wild type enzyme, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 0.0971
-
 5,5'-dithiobis(2-nitrobenzoic acid) mutant enzyme Y116I, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 2.89
-
 Lipoamide wild type enzyme, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 3.68
-
 Lipoamide mutant enzyme Y116I, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 5.59
-
 Lipoamide mutant enzyme Y116T, in TE buffer, at 20°C Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.1.9 selenium thioredoxin reductase 1 is a selenoprotein, the oxidized enzyme presents a selenenylsulfide motif in trans-configuration, with the selenium atom of selenocysteine-498 positioned beneath the side chain of Tyr-116 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Rattus norvegicus O89049
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.9 2',5'-ADP Sepharose column chromatography and phenylarsine oxide-Sepharose column chroamtography Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Rattus norvegicus 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.9 5-hydroxy-1,4-naphthoquinone + NADPH + H+
-
 Rattus norvegicus ?
-
 ?
1.8.1.9 lipoamide + NADPH + H+
-
 Rattus norvegicus dihydrolipoamide + NADP+
-
 ?
1.8.1.9 thioredoxin + insulin disulfide thioredixin is the native principal substrate of TrxR1 Rattus norvegicus thioredoxin disulfide + insulin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.9 thioredoxin reductase 1
-
 Rattus norvegicus
1.8.1.9 TrxR1
-
 Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.9 2 3.7 Lipoamide wild type enzyme, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 24.1
-
 5-hydroxy-1,4-naphthoquinone wild type enzyme, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 27.6
-
 Lipoamide mutant enzyme Y116I, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 31.2
-
 Lipoamide mutant enzyme Y116T, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 40.98
-
 thioredoxin disulfide mutant enzyme Y116I, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 44.85
-
 thioredoxin disulfide mutant enzyme Y116T, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 47.72
-
 5,5'-dithiobis(2-nitrobenzoic acid) mutant enzyme Y116T, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 49.87
-
 5,5'-dithiobis(2-nitrobenzoic acid) mutant enzyme Y116I, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 52.75
-
 5-hydroxy-1,4-naphthoquinone mutant enzyme Y116T, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 70.3
-
 5,5'-dithiobis(2-nitrobenzoic acid) wild type enzyme, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 94.17
-
 thioredoxin disulfide wild type enzyme, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 174.3
-
 5-hydroxy-1,4-naphthoquinone mutant enzyme Y116I, in TE buffer, at 20°C Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 FAD contains 6 FAD molecules Rattus norvegicus
1.8.1.9 NADP+ contains 6 NADP+ molecules Rattus norvegicus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.1.9 5.6
-
 Lipoamide mutant enzyme Y116T, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 7.5
-
 Lipoamide mutant enzyme Y116I, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 513.3
-
 5,5'-dithiobis(2-nitrobenzoic acid) mutant enzyme Y116I, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 526.7
-
 5,5'-dithiobis(2-nitrobenzoic acid) mutant enzyme Y116T, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 748.3
-
 5,5'-dithiobis(2-nitrobenzoic acid) wild type enzyme, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 7343
-
 5-hydroxy-1,4-naphthoquinone mutant enzyme Y116I, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 7557
-
 5-hydroxy-1,4-naphthoquinone mutant enzyme Y116T, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 10620
-
 5-hydroxy-1,4-naphthoquinone wild type enzyme, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 41450
-
 thioredoxin disulfide mutant enzyme Y116I, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 42150
-
 thioredoxin disulfide mutant enzyme Y116T, in TE buffer, at 20°C Rattus norvegicus
1.8.1.9 65940
-
 thioredoxin disulfide wild type enzyme, in TE buffer, at 20°C Rattus norvegicus