BRENDA - Enzyme Database

The catalytic mechanism of Sulfiredoxin from Saccharomyces cerevisiae passes through an oxidized disulfide Sulfiredoxin intermediate that is reduced by thioredoxin

Roussel, X.; Kriznik, A.; Richard, C.; Rahuel-Clermont, S.; Branlant, G.; J. Biol. Chem. 284, 33048-33055 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.8.98.2
-
Saccharomyces cerevisiae
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.8.98.2
C106A
mutant, constructed to address questions regarding the catalytic mechanisms and the role of the cysteine residues
Saccharomyces cerevisiae
1.8.98.2
C48A
mutant, constructed to address questions regarding the catalytic mechanisms and the role of the cysteine residues
Saccharomyces cerevisiae
1.8.98.2
C48A/C106A
mutant, constructed to address questions regarding the catalytic mechanisms and the role of the cysteine residues
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.98.2
15000
-
Srx monomer, determined by SDS-PAGE
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + R-SH
Saccharomyces cerevisiae
-
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.98.2
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.98.2
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + R-SH
-
698953
Saccharomyces cerevisiae
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.8.98.2
ATP
-
Saccharomyces cerevisiae
1.8.98.2
dithiothreitol
-
Saccharomyces cerevisiae
1.8.98.2
thioredoxin
-
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.98.2
-
Saccharomyces cerevisiae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.8.98.2
ATP
-
Saccharomyces cerevisiae
1.8.98.2
dithiothreitol
-
Saccharomyces cerevisiae
1.8.98.2
thioredoxin
-
Saccharomyces cerevisiae
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.8.98.2
C106A
mutant, constructed to address questions regarding the catalytic mechanisms and the role of the cysteine residues
Saccharomyces cerevisiae
1.8.98.2
C48A
mutant, constructed to address questions regarding the catalytic mechanisms and the role of the cysteine residues
Saccharomyces cerevisiae
1.8.98.2
C48A/C106A
mutant, constructed to address questions regarding the catalytic mechanisms and the role of the cysteine residues
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.98.2
15000
-
Srx monomer, determined by SDS-PAGE
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + R-SH
Saccharomyces cerevisiae
-
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.98.2
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + R-SH
-
698953
Saccharomyces cerevisiae
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R
-
-
-
?