Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tarrago, L.; Laugier, E.; Zaffagnini, M.; Marchand, C.; Le Marechal, P.; Rouhier, N.; Lemaire, S.D.; Rey, P.
    Regeneration mechanisms of Arabidopsis thaliana methionine sulfoxide reductases B by glutaredoxins and thioredoxins (2009), J. Biol. Chem., 284, 18963-18971.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.12 expressed in Escherichia coli M15rep4 cells Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.8.4.12 C116S MSRB1 mutant with decreased kcat values for dabsyl-L-methionine (R)-sulfoxide compared to the wild type enzyme Arabidopsis thaliana
1.8.4.12 C134T MSRB2 mutant with increased kcat values for dabsyl-L-methionine (R)-sulfoxide compared to the wild type enzyme Arabidopsis thaliana
1.8.4.12 T132A MSRB1 mutant with decreased kcat values for dabsyl-L-methionine (R)-sulfoxide compared to the wild type enzyme Arabidopsis thaliana
1.8.4.12 T132C MSRB1 mutant with decreased kcat values for dabsyl-L-methionine (R)-sulfoxide compared to the wild type enzyme Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.4.12 0.0068
-
 glutaredoxin C4 isoform MSRB1 wild type enzyme Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.4.12 plastid
-
 Arabidopsis thaliana 9536
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.4.12 16610
-
 reduced isoform MSRB1, MALDI-TOF mass spectrometry Arabidopsis thaliana
1.8.4.12 16620
-
 reduced isoform MSRB1, calculated from amino acid sequence Arabidopsis thaliana
1.8.4.12 16860
-
 reduced isoform MSRB2, calculated from amino acid sequence Arabidopsis thaliana
1.8.4.12 16860
-
 reduced isoform MSRB2, MALDI-TOF mass spectrometry Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.12 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.12 HiTrap column chromatography and Sephadex G-25 gel filtration Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.12 dabsyl-L-methionine (R)-sulfoxide + glutaredoxin C4
-
 Arabidopsis thaliana ?
-
 ?
1.8.4.12 dabsyl-L-methionine (R)-sulfoxide + glutaredoxin S12
-
 Arabidopsis thaliana ?
-
 ?
1.8.4.12 dabsyl-L-methionine (R)-sulfoxide + thioredoxin
-
 Arabidopsis thaliana dabsyl-L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.12 dabsyl-L-methionine (R)-sulfoxide + thioredoxin h1
-
 Arabidopsis thaliana ?
-
 ?
1.8.4.12 L-methionine (R)-sulfoxide + thioredoxin
-
 Arabidopsis thaliana L-methionine + thioredoxin disulfide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.4.12 methionine sulfoxide reductase B
-
 Arabidopsis thaliana
1.8.4.12 MSRB1 isoform Arabidopsis thaliana
1.8.4.12 MsrB2 isoform Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.4.12 0.002
-
 dabsyl-L-methionine (R)-sulfoxide isoform MSRB1 wild type enzyme, in the presence of 20 mMTris(2-carboxyethyl) phosphine hydrochloride Arabidopsis thaliana
1.8.4.12 0.005
-
 dabsyl-L-methionine (R)-sulfoxide isoform MSRB1 mutant T132C, in the presence of 20 mM dithioerythritol Arabidopsis thaliana
1.8.4.12 0.012
-
 dabsyl-L-methionine (R)-sulfoxide isoform MSRB1 mutant T132A, in the presence of 20 mM dithioerythritol Arabidopsis thaliana
1.8.4.12 0.014
-
 dabsyl-L-methionine (R)-sulfoxide isoform MSRB2 wild type enzyme, in the presence of 20 mMTris(2-carboxyethyl) phosphine hydrochloride Arabidopsis thaliana
1.8.4.12 0.02
-
 glutaredoxin C4 isoform MSRB2 wild type enzyme Arabidopsis thaliana
1.8.4.12 0.028
-
 dabsyl-L-methionine (R)-sulfoxide isoform MSRB2 wild type enzyme, in the presence of 20 mM dithioerythritol Arabidopsis thaliana
1.8.4.12 0.03
-
 glutaredoxin S12 isoform MSRB2 wild type enzyme Arabidopsis thaliana
1.8.4.12 0.047
-
 dabsyl-L-methionine (R)-sulfoxide isoform MSRB1 mutant C116S, in the presence of 20 mM dithioerythritol Arabidopsis thaliana
1.8.4.12 0.064
-
 dabsyl-L-methionine (R)-sulfoxide isoform MSRB2 mutant C134T, in the presence of 20 mM dithioerythritol Arabidopsis thaliana
1.8.4.12 0.075
-
 dabsyl-L-methionine (R)-sulfoxide isoform MSRB1 wild type enzyme, in the presence of 20 mM dithioerythritol Arabidopsis thaliana
1.8.4.12 0.3
-
 glutaredoxin C4 isoform MSRB2 mutant enzyme C134T Arabidopsis thaliana
1.8.4.12 0.31
-
 glutaredoxin S12 isoform MSRB1 mutant enzyme T132A Arabidopsis thaliana
1.8.4.12 0.34
-
 glutaredoxin C4 isoform MSRB1 mutant enzyme C116S Arabidopsis thaliana
1.8.4.12 0.36
-
 glutaredoxin S12 isoform MSRB2 mutant enzyme C134T Arabidopsis thaliana
1.8.4.12 0.37
-
 glutaredoxin C4 isoform MSRB1 mutant enzyme T132A Arabidopsis thaliana
1.8.4.12 0.48
-
 glutaredoxin C4 isoform MSRB1 wild type enzyme Arabidopsis thaliana
1.8.4.12 0.5
-
 glutaredoxin S12 isoform MSRB1 mutant enzyme C116S Arabidopsis thaliana
1.8.4.12 0.58
-
 glutaredoxin S12 isoform MSRB1 wild type enzyme Arabidopsis thaliana
1.8.4.12 1.28
-
 thioredoxin h1 isoform MSRB2 wild type enzyme Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.4.12 thioredoxin
-
 Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.4.12 73.4
-
 glutaredoxin C4 isoform MSRB1 wild type enzyme Arabidopsis thaliana